Liquid Chromatographic Determination of Rifampin In Human Plasma, Bronchoalveolar Lavage Fluid, and Alveolar Cells

• Published in: Journal of chromatographic science Vol. 38; no. 2; pp. 72 - 76
• Main Authors: Conte, John E., Lin, Emil, Zurlinden, Elisabeth
• Format: Journal Article
• Language: English
• Published: Niles, IL Oxford University Press 01-02-2000; Preston Publications
• Subjects: Analysis; Anti-Anxiety Agents - blood; Antibiotics, Antitubercular - blood; Biological and medical sciences; Bronchoalveolar Lavage Fluid - chemistry; Chromatography, High Pressure Liquid; Diazepam - blood; General pharmacology; Humans; Medical sciences; Pharmacology. Drug treatments; Pulmonary Alveoli - chemistry; Pulmonary Alveoli - cytology; Reproducibility of Results; Rifampin - blood; Solutions; Biological fluid; Pneumocyte; Fluorescence detector; Chemical analysis; Lung; HPLC chromatography; Blood; Blood plasma; Antibiotic; Reversed phase chromatography; Sample preparation; Rifampicin; Bronchoalveolar lavage; Antituberculous agent; Antibacterial agent; Quantitative analysis
• ISSN: 0021-9665; 1945-239X
• DOI: 10.1093/chromsci/38.2.72

A technique is presented for the measurement of rifampin in humans by reversed-phase column chromatography and postcolumn photo irradiation. The assay employs diazepam as an internal standard and provides specific, rapid; and reliable determinations for drug concentration in plasma, bronchoalveolar lavage (BAL), and alveolar cells (AC). The preparation of plasma and AC samples utilizes a simple deproteinization step, whereas BAL supernatants require a solid-phase extraction. The assay produces sharp peaks with retention times of 6.2 and 15 min for rifampin and diazepam, respectively. The detection limits for rifampin were 0.5 µg/mL for plasma, 0.015 µg/mL for BAL supernatants, and 0.03 µg/mL for AC suspensions. The assay has excellent performance characteristics, making it suitable for pharmacological studies in humans, and it is being used to support a study of the intrapulmonary pharmacokinetics of rifampin.