In-house validation of a multiplex real-time PCR method for simultaneous detection of Salmonella spp., Escherichia coli O157 and Listeria monocytogenes

A wide variety of qPCR methods currently exist for Salmonella spp., Escherichia coli O157 and Listeria monocytogenes detection. These methods target several genes and use different detection chemistries, either in simplex or in multiplex formats. However, the majority of these methods have not been...

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Bibliographic Details
Published in:	International journal of food microbiology Vol. 164; no. 1; pp. 92 - 98
Main Authors:	Garrido, Alejandro, Chapela, María-José, Román, Belén, Fajardo, Paula, Vieites, Juan M., Cabado, Ana G.
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 03-06-2013 Elsevier
Subjects:	Biological and medical sciences Carbohydrate Epimerases - genetics Culture Media DNA Primers E. coli O157 Escherichia coli Escherichia coli O157 - genetics Escherichia coli O157 - physiology Food industries Food microbiology Food Microbiology - methods Fundamental and applied biological sciences. Psychology General aspects L. monocytogenes Listeria monocytogenes Listeria monocytogenes - genetics Listeria monocytogenes - physiology Methods of analysis, processing and quality control, regulation, standards Multiplex Polymerase Chain Reaction - standards Multiplex qPCR Real-Time Polymerase Chain Reaction - standards Salmonella Salmonella - genetics Salmonella - physiology Salmonella spp Sensitivity and Specificity Transaminases - genetics Validation Validation E. coli O157 L. monocytogenes Salmonella spp Multiplex qPCR Salmonella Escherichia coli Listeria monocytogenes Real time Polymerase chain reaction Analysis method Bacteria Control method Molecular biology Detection Enterobacteriaceae
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