Linkage analysis of er-1, a recessive Pisum sativum gene for resistance to powdery mildew fungus (Erysiphe pisi D.C.)

• Published in: Theoretical and applied genetics Vol. 88; no. 8; pp. 1050 - 1055
• Main Authors: Timmerman, G.M, Frew, T.J, Weeden, N.F, Miller, A.L, Goulden, S.S. (New Zealand Inst. for Crop and Food Research Ltd., Christchurch (New Zealand))
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01-09-1994; Berlin
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; CARTE GENETIQUE; Classical genetics, quantitative genetics, hybrids; ERYSIPHE PISI; Fundamental and applied biological sciences. Psychology; GENE RECESSIF; GENES RECESIVOS; GENETICA MOLECULAR; Genetics of eukaryotes. Biological and molecular evolution; GENETIQUE MOLECULAIRE; GENOMAS; GENOME; MAPAS GENETICOS; PISUM SATIVUM; Pteridophyta, spermatophyta; RESISTANCE AUX MALADIES; RESISTENCIA A LA ENFERMEDAD; Vegetals; Genetic mapping; Genetic inheritance; Linkage; Genetic marker; Fungus resistance; Fungi; Grain legume; Leguminosae; Erysiphe pisi; Investigation method; Random amplified polymorphic DNA; Dicotyledones; Pisum sativum; Angiospermae; Ascomycetes; Spermatophyta; Polymorphism; Thallophyta
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/bf00220815

Linkage analysis was used to determine the genetic map location of er-1, a recessive gene conditioning resistance to powdery mildew, on the Pisum sativum genome. Genetic linkage was demonstrated between er-1 and linkage group 6 markers after analyzing the progeny of two crosses, an F2 population and a set of recombinant inbred lines. The classes of genetic markers surrounding er-1 include RFLP, RAPD and allozyme markers as well as the morphological marker Gty. A RAPD marker tightly linked to er-1 was identified by bulked segregant analysis. After DNA sequence characterization, specific PCR primers were designed to convert this RAPD marker into a sequence characterized amplified region (SCAR).