Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane

• Published in: Biotechnology letters Vol. 26; no. 7; pp. 563 - 567
• Main Authors: RAMACHANDRA MURTY, V, BHAT, Jayadev, MUNISWARAN, P. K. A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-04-2004; Springer Nature B.V
• Subjects: Batch reactors; Biological and medical sciences; Bioreactors; Biotechnology; Candida - enzymology; Candida rugosa; Enzyme Stability; Enzymes, Immobilized - chemistry; Fundamental and applied biological sciences. Psychology; Hydrolysis; Kinetics; Lipase - chemistry; Models, Chemical; Octanes - chemistry; Oryza sativa; Plant Oils - chemistry; Reactors; Rice; Rice Bran Oil; Packed bed; Stability; Enzyme; Immobilization; Triacylglycerol lipase; Esterases; Isooctane; Carboxylic ester hydrolases; Fungi; Hydrolysis; Hydrolases; Fungi Imperfecti; Rice bran oil; Candida rugosa; Thallophyta
• ISSN: 0141-5492; 1573-6776
• DOI: 10.1023/B:BILE.0000021956.33855.11

The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, K1, of 0.92 M. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life was 360 h without isooctane but 288 h with 25% (v/v) isooctane in rice bran oil.