Lactiplantibacillus plantarum and Saussurea costus as Therapeutic Agents against a Diabetic Rat Model-Approaches to Investigate Pharmacophore Modeling of Human IkB Kinase and Molecular Interaction with Dehydrocostus Lactone of Saussurea costus

• Published in: Metabolites Vol. 13; no. 6; p. 764
• Main Authors: AlGeffari, Metab A, Mansour, Dina, Ahmed-Farid, Omar, Mohamed Yousef, Einas, Mohamed, Shereen A, Moustafa, Mahmoud M A, Barakat, Hassan, Abd El Ghany, Khalid
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19-06-2023; MDPI
• Subjects: 16S rRNA gene; Acids; Animals; Aqueous solutions; Arteriosclerosis; Bacteria; Beta protein; biochemical; Diabetes; Diabetes mellitus (non-insulin dependent); Down-regulation; Herbal medicine; histological analysis; Interleukin 17; Interleukin 6; Kinases; Laboratories; Lactic acid bacteria; Lactiplantibacillus plantarum; MAP kinase; Maximum likelihood method; Metabolism; Metabolites; NF-κB protein; pharmacophore modeling; Saussurea costus; Thermal cycling; TRAF6 protein; Tumor necrosis factor-α; St Louis Missouri; United States > US; India; docking; Saussurea costus; biochemical; pharmacophore modeling; diabetes mellitus; histological analysis; 16S rRNA gene; Lactiplantibacillus plantarum
• ISSN: 2218-1989; 2218-1989
• DOI: 10.3390/metabo13060764

Lactic acid bacteria is well-known as a vital strategy to alleviate or prevent diabetes. Similarly, the plant
(Falc) Lipsch is a preventive power against diabetes. Here, we aimed to determine whether lactic acid bacteria or
is more effective in treating a diabetic rat model in a comparative study manner. An in vivo experiment was conducted to test the therapeutic activity of
(MW719476.1) and
plants against an alloxan-induced diabetic rat model. Molecular, biochemical, and histological analyses were investigated to evaluate the therapeutic characteristics of different treatments. The high dose of
revealed the best downregulated expression for the
,
,
,
,
,
,
,
,
, and
genes compared to
and the control groups. The downregulation of
by
could be attributed to dehydrocostus lactone as an active compound with proposed antidiabetic activity. So, we performed another pharmacophore modeling analysis to test the possible interaction between human IkB kinase beta protein and dehydrocostus lactone as an antidiabetic drug. Molecular docking and MD simulation data confirmed the interaction between human IkB kinase beta protein and dehydrocostus lactone as a possible drug. The target genes are important in regulating type 2 diabetes mellitus signaling, lipid and atherosclerosis signaling, NF-κB signaling, and IL-17 signaling pathways. In conclusion, the
plant could be a promising source of novel therapeutic agents for treating diabetes and its complications. Dehydrocostus lactone caused the ameliorative effect of
by its interaction with human IkB kinase beta protein. Further, future studies could be conducted to find the clinical efficacy of dehydrocostus lactone.