Protective effect of Chresta martii extract on the zymosan-induced temporomandibular joint arthritis in rats

• Published in: Journal of oral biology and craniofacial research (Amsterdam) Vol. 10; no. 3; pp. 276 - 280
• Main Authors: Rocha do Val, Danielle, Bezerra, Mirna Marques, Fernandes Gomes, Francisco Isaac, Nobre, Christiane Aguiar, Teixeira, Suzana Capistrano, Lemos, Jonas Cavalcante, Alves Pereira, Karuza Maria, de Paulo Teixeira Pinto, Vicente, Rodrigues e Silva, Antônio Alfredo, de Sousa Franco, Eryvelton, Bernadete de Sousa Maia, Maria, Chaves, Hellíada Vasconcelos
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-07-2020; Elsevier
• Subjects: Arthritis; Chresta martii; Heme oxygenase-1; Research Paper; Temporomandibular joint; Tumor necrosis factor-α; Temporomandibular joint; Arthritis; Tumor necrosis factor-α; Chresta martii; Heme oxygenase-1
• ISSN: 2212-4268; 2212-4276
• DOI: 10.1016/j.jobcr.2020.05.005

Chresta martii is broadly used by folk medicine due to its anti-inflammatory effects, but there is a lack of preclinical data on its pharmacological mechanisms. This study investigated the efficacy of Chresta martii ethanolic extract (CEE) in the zymosan-induced temporomandibular joint arthritis (TMJ) and evaluated the possible role of TNF-α, nitric oxide (NO), and heme oxygenase-1 (HO-1).
Male Wistar rats (160–220 g) were pre-treated with CEE (100, 200 or 400 mg/kg; v.o) 1 h before zymosan injection (2 mg; i.art). Mechanical hypernociception (g) was assessed 4 h later. The trigeminal ganglion was collected for TNF-α quantification (ELISA), total cell count and myeloperoxidase activity (MPO) were assayed in the synovial lavage 6 h after arthritis induction. Additionally, animals were pre-treated with L-NAME (30 mg/kg; i.p.) or ZnPP-IX (3 mg/kg, s.c.) to assess the involvement of NO and HO-1, respectively.
CEE 400 mg/kg (v.o) increased (p < 0.05) hypernociception threshold, reduced the cell counts and MPO activity in the synovial lavage, as well as decreased TNF-α levels in the trigeminal ganglion. ZnPP-IX abolished the analgesic effect of CEE, but not L-NAME.
The anti-inflammatory and antinociceptive effects of CEE depended on the HO-1 pathway integrity and TNF-α suppression.