gap-filling sequence on the left arm of chromosome 2 in fission yeast Schizosaccharomyces pombe

• Published in: Yeast (Chichester, England) Vol. 25; no. 9; pp. 673 - 679
• Main Authors: Sasaki, Mayumi, Idiris, Alimjan, Tada, Aya, Kumagai, Hiromichi, Giga-Hama, Yuko, Tohda, Hideki
• Format: Journal Article
• Language: English
• Published: Chichester, UK John Wiley & Sons, Ltd 01-09-2008
• Subjects: Amino Acid Motifs; amino acid permease; Amino Acid Sequence; Base Sequence; Chromosomes, Fungal; DNA, Fungal - chemistry; DNA, Fungal - genetics; gap‐filling sequence; Gene Library; glutathione S‐transferase C‐terminal domain; major facilitator superfamily; Molecular Sequence Data; Open Reading Frames; Polymerase Chain Reaction; Schizosaccharomyces - genetics; Schizosaccharomyces pombe; Sequence Alignment; taurine catabolism dioxygenase TauD TfdA family
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/yea.1613

We report a gap-filling sequence between SPBPB21E7.09 (in contig c1348) and SPBPB10D8.01 (in contig pB10D8) on the left arm of chromosome 2 in the fission yeast, Schizosaccharomyces pombe. The sequence was determined from a BAC clone overlapping SPBPB21E7.01c (eno102) (in contig c1348) and SPBC1683.07 (mal1) (in contig pB10D8). The gap-filling sequence is 17 881 bp in length and contains five putative open reading frames, which were systematically named as SPBC460.01c, SPBC460.02c, SPBC460.03, SPBC460.04c and SPBC460.05. Their deduced amino acid sequences respectively include protein motifs corresponding to amino acid permease, glutathione S-transferase C-terminal domain, taurine catabolism dioxygenase TauD TfdA family and major facilitator superfamily, whereas their functions are unknown. The sequence has been submitted to the international DNA database (DDBJ/EMBL/GenBank) under Accession No. AB325691. Copyright © 2008 John Wiley & Sons, Ltd.