ATP-binding cassette (ABC) transporters in caprine preantral follicles: gene and protein expression

• Published in: Cell and tissue research Vol. 372; no. 3; pp. 611 - 620
• Main Authors: Guerreiro, Denise Damasceno, de Lima, Laritza Ferreira, Mbemya, Gildas Tetaping, Maside, Carolina Mielgo, Miranda, André Marrocos, Tavares, Kaio César Simiano, Alves, Benner Geraldo, Faustino, Luciana Rocha, Smitz, Johan, de Figueiredo, José Ricardo, Rodrigues, Ana Paula Ribeiro
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-06-2018; Springer; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biomedicine; Blood-brain barrier; Brain stem; Detoxification; Follicles; Gene expression; Goats; Human Genetics; Immunofluorescence; Intestine; Liver; Localization; Microbial drug resistance; Molecular Medicine; mRNA; Multidrug resistance; Multidrug resistant organisms; Placenta; Protein expression; Proteins; Proteomics; Regular Article; RNA; Stem cells; Preantral follicles; ABCB1; ABCC2; Multidrug resistance; ABCG2
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-018-2804-3

The multidrug resistance proteins ABCB1, ABCC2 and ABCG2 are an energy-dependent efflux pump that functions in systemic detoxification processes. Physiologically expressed in a variety of tissues, most abundantly in the liver and intestinal epithelia, placenta, blood-brain barrier and various stem cells, until now, these pumps were not identified in goat ovarian tissue. Therefore, the aim of this study is to analyze ABCB1, ABCC2, and ABCG2 mRNA and protein expression in goat preantral follicles. Fragments (3 × 3 × 1 mm) from five pairs of ovary (
n
 = 10) obtained from five goat were collected and immediately submitted to qPCR, Western blot, and immunofluorescence assay for mRNA detection and identification and localization of the ABC transporters, respectively. mRNA for
ABCB1
,
ABCC2
, and
ABCG2
and the presence of their proteins were observed on ovarian tissue samples. Positive marks were observed for the three transport proteins in all follicular categories studied. However, the marks were primarily localized in the oocyte of primordial, transition and primary follicle categories. In conclusion, goat ovarian tissue expresses mRNA for the ABCB1, ABCC2 and ABCG2 transporters and the expression of these proteins in the preantral follicles is a follicle-dependent stage.