Sonic hedgehog pathway activation increases mitochondrial abundance and activity in hippocampal neurons

• Published in: Molecular biology of the cell Vol. 28; no. 3; pp. 387 - 395
• Main Authors: Yao, Pamela J, Manor, Uri, Petralia, Ronald S, Brose, Rebecca D, Wu, Ryan T Y, Ott, Carolyn, Wang, Ya-Xian, Charnoff, Ari, Lippincott-Schwartz, Jennifer, Mattson, Mark P
• Format: Journal Article
• Language: English
• Published: United States The American Society for Cell Biology 01-02-2017
• Subjects: Animals; Brief Reports; Cell Culture Techniques; GTP Phosphohydrolases - metabolism; Hedgehog Proteins - metabolism; HEK293 Cells; Hippocampus - metabolism; Humans; Mitochondria - metabolism; Mitochondria - physiology; Mitochondrial Proteins - metabolism; Neurons - metabolism; Neurons - physiology; Rats; Signal Transduction - physiology
• ISSN: 1059-1524; 1939-4586
• DOI: 10.1091/mbc.E16-07-0553

Mitochondria are essential organelles whose biogenesis, structure, and function are regulated by many signaling pathways. We present evidence that, in hippocampal neurons, activation of the Sonic hedgehog (Shh) signaling pathway affects multiple aspects of mitochondria. Mitochondrial mass was increased significantly in neurons treated with Shh. Using biochemical and fluorescence imaging analyses, we show that Shh signaling activity reduces mitochondrial fission and promotes mitochondrial elongation, at least in part, via suppression of the mitochondrial fission protein dynamin-like GTPase Drp1. Mitochondria from Shh-treated neurons were more electron-dense, as revealed by electron microscopy, and had higher membrane potential and respiratory activity. We further show that Shh protects neurons against a variety of stresses, including the mitochondrial poison rotenone, amyloid β-peptide, hydrogen peroxide, and high levels of glutamate. Collectively our data suggest a link between Shh pathway activity and the physiological properties of mitochondria in hippocampal neurons.