Magnetic particles for in vitro molecular diagnosis: From sample preparation to integration into microsystems

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 158; pp. 1 - 8
• Main Authors: Tangchaikeeree, Tienrat, Polpanich, Duangporn, Elaissari, Abdelhamid, Jangpatarapongsa, Kulachart
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-10-2017; Elsevier
• Subjects: Biosensing Techniques - methods; Lab-On-A-Chip Devices; Lab-on-a-chip microsystem; Life Sciences; Magnetic particles; Magnetics - methods; Microfluidic Analytical Techniques - methods; Microfluidic biosensor; Nucleic acids; Pharmaceutical sciences; Polymerase chain reaction; Polymerase chain reaction; Magnetic particles; Microfluidic biosensor; Nucleic acids; Lab-on-a-chip microsystem
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2017.06.024

Versatility of magnetic nanoparticles in in vitro molecular diagnostics.
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•Development of specific immobilization of colloidal magnetic particles for rapid separation of target molecules.•Functionalization of colloidal magnetic particles for molecular detection and readout system.•Application of colloidal magnetic particles in microfluidic and lab-on-a-chip biosensor.
Colloidal magnetic particles (MPs) have been developed in association with molecular diagnosis for several decades. MPs have the great advantage of easy manipulation using a magnet. In nucleic acid detection, these particles can act as a capture support for rapid and simple biomolecule separation. The surfaces of MPs can be modified by coating with various polymer materials to provide functionalization for different applications. The use of MPs enhances the sensitivity and specificity of detection due to the specific activity on the surface of the particles. Practical applications of MPs demonstrate greater efficiency than conventional methods. Beyond traditional detection, MPs have been successfully adopted as a smart carrier in microfluidic and lab-on-a-chip biosensors. The versatility of MPs has enabled their integration into small single detection units. MPs-based biosensors can facilitate rapid and highly sensitive detection of very small amounts of a sample. In this review, the application of MPs to the detection of nucleic acids, from sample preparation to analytical readout systems, is described. State-of-the-art integrated microsystems containing microfluidic and lab-on-a-chip biosensors for the nucleic acid detection are also addressed.