Impact of BMI and Cardiorespiratory Fitness on Oxidative Stress in Plasma and Circulating Exosomes Following Acute Exercise

• Published in: Biology (Basel, Switzerland) Vol. 13; no. 8; p. 599
• Main Authors: Slusher, Aaron L, Visavadiya, Nishant P, Fico, Brandon G, Estébanez, Brisamar, Acevedo, Edmund O, Huang, Chun-Jung
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08-08-2024; MDPI
• Subjects: aerobic exercise; Aerobics; Body fat; Body mass index; Cardiorespiratory fitness; Cardiovascular disease; Diabetes; Ethylenediaminetetraacetic acid; Exercise; Exercise equipment; exercise secretome; Exosomes; Extracellular vesicles; Fitness equipment; Glutathione; Laboratories; Metabolism; Muscles; Musculoskeletal system; Obesity; Oxidative stress; Physical activity; Physical fitness; Plasma; Running; Scientific equipment and supplies industry; Skeletal muscle; Stress response; United States; United States > US; aerobic exercise; exercise secretome; body mass index; physical activity
• ISSN: 2079-7737; 2079-7737
• DOI: 10.3390/biology13080599

The impact of cardiorespiratory fitness (VO
) and obesity on indices of oxidative stress in plasma and circulating exosome-like extracellular vesicles (ELVs) were examined following acute exercise. Indices of oxidative stress in plasma and isolated plasma ELVs were examined in aerobically trained (NW-Tr; n = 15) and untrained (NW-UTr; n = 18) normal-weight individuals and aerobically untrained individuals with obesity (Ob-Utr; n = 10) prior to and immediately following acute maximal treadmill running. Following exercise, ELV flotillin-1 expression (
= 0.008) and plasma total antioxidant capacity (TAC;
= 0.010) increased more in NW-UTr compared to NW-Tr and Ob-UTr participants, whereas plasma protein carbonyls (PC) decreased more in Ob-UTr compared to NW-Tr and NW-UTr groups. ELV glutathione (GSH) concentrations decreased more in NW-Tr compared to NW-UTr and Ob-UTr participants (
= 0.009), whereas lipid peroxidase (LPO) concentrations increased more in Ob-UTr compared to NW-Tr and NW-UTr participants (
= 0.003). Body mass index (BMI) was associated negatively with plasma TAC and PC (
< 0.05) and positively with ELV LPO concentration responses (
= 0.009). Finally, plasma-to-total (plasma + ELV) GSH ratios decreased in Ob-UTr compared to NW-Tr and NW-UTr participants (
= 0.006), PC ratios increased in NW-Tr and NW-UTr compared to Ob-UTr subjects (
= 0.008), and reactive oxygen/nitrogen species ratios increased in NW-UTr and decreased in Ob-UTr participants (
< 0.001). BMI, independently of VO
, differentially regulates indices of oxidative stress within plasma and circulating ELVs prior to and immediately following acute maximal treadmill exercise.