recA mutations reduce adherence and colonization by classical and El Tor strains of Vibrio cholerae
Division of Microbial Genetics, Central Drug Research Institute, Lucknow 226001, India Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, MD 21201, USA Author for correspondence: Brahm S. Srivastava. Tel: +91 522 232411. Fax: +91 522 243405. ABSTRACT Two recA muta...
Saved in:
Published in: | Microbiology (Society for General Microbiology) Vol. 140; no. 5; pp. 1217 - 1222 |
---|---|
Main Authors: | , , , , , |
Format: | Journal Article |
Language: | English |
Published: |
Reading
Soc General Microbiol
01-05-1994
Society for General Microbiology |
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Summary: | Division of Microbial Genetics, Central Drug Research Institute, Lucknow 226001, India
Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, MD 21201, USA
Author for correspondence: Brahm S. Srivastava. Tel: +91 522 232411. Fax: +91 522 243405.
ABSTRACT
Two recA mutants of Vibrio cholerae (classical and El Tor biotypes) were constructed by disruption of the wild-type recA gene with mutated recA sequences of V. cholerae cloned in the suicide vector pGP704. Mutants defective in the recA gene were compared with their respective RecA + parent strains with regard to their adherence to isolated rabbit intestine and colonization of intestine of infant mice. The recA mutation in V. cholerae was found to diminish adherence and markedly affected colonization.
Keywords: Vibrio cholerae , recA , adherence, colonization |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1350-0872 1465-2080 |
DOI: | 10.1099/13500872-140-5-1217 |