recA mutations reduce adherence and colonization by classical and El Tor strains of Vibrio cholerae

Division of Microbial Genetics, Central Drug Research Institute, Lucknow 226001, India Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, MD 21201, USA Author for correspondence: Brahm S. Srivastava. Tel: +91 522 232411. Fax: +91 522 243405. ABSTRACT Two recA muta...

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Published in:Microbiology (Society for General Microbiology) Vol. 140; no. 5; pp. 1217 - 1222
Main Authors: Kumar, K. Krishna, Srivastava, Ranjana, Sinha, V. B, Michalski, Jane, Kaper, James B, Srivastava, Brahm S
Format: Journal Article
Language:English
Published: Reading Soc General Microbiol 01-05-1994
Society for General Microbiology
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Summary:Division of Microbial Genetics, Central Drug Research Institute, Lucknow 226001, India Center for Vaccine Development, University of Maryland, School of Medicine, Baltimore, MD 21201, USA Author for correspondence: Brahm S. Srivastava. Tel: +91 522 232411. Fax: +91 522 243405. ABSTRACT Two recA mutants of Vibrio cholerae (classical and El Tor biotypes) were constructed by disruption of the wild-type recA gene with mutated recA sequences of V. cholerae cloned in the suicide vector pGP704. Mutants defective in the recA gene were compared with their respective RecA + parent strains with regard to their adherence to isolated rabbit intestine and colonization of intestine of infant mice. The recA mutation in V. cholerae was found to diminish adherence and markedly affected colonization. Keywords: Vibrio cholerae , recA , adherence, colonization
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ISSN:1350-0872
1465-2080
DOI:10.1099/13500872-140-5-1217