Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook

• Published in: Brazilian Journal of Pharmaceutical Sciences Vol. 53; no. 1
• Main Authors: Emir, Ahmet, Emir, Ceren, Bozkurt, Buket, Onur, Mustafa Ali, Somer, Nehir Unver, Kaya, Gulen Irem
• Format: Journal Article
• Language: English
• Published: Sao Paulo Universidade de Sao Paulo Faculdade de Ciencias 01-01-2017; Universidade de São Paulo, Faculdade de Ciências Farmacêuticas; Universidade de São Paulo
• Subjects: Accuracy; Alkaloids; Alzheimer's disease; Amaryllidaceae; Chromatography; High Perfomance Liquid Chromatography/quantitative analysis; High Performance Liquid Chromatography/method validation; PHARMACOLOGY & PHARMACY; Pharmacy; Phytochemicals; Plant extracts; Quantitative analysis; Sensors; Validation studies; Turkey; Alkaloids; High Perfomance Liquid Chromatography/quantitative analysis; Amaryllidaceae; High Performance Liquid Chromatography/method validation; Plant extracts
• ISSN: 1984-8250; 2175-9790; 1984-8250; 2175-9790
• DOI: 10.1590/s2175-97902017000115063

In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.01:92.5:7.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.