Beta-carotene preferentially regulates chicken myoblast proliferation withdrawal and differentiation commitment via BCO1 activity and retinoic acid production

• Published in: Experimental cell research Vol. 358; no. 2; pp. 140 - 146
• Main Authors: Praud, C., Al Ahmadieh, S., Voldoire, E., Le Vern, Y., Godet, E., Couroussé, N., Graulet, B., Le Bihan Duval, E., Berri, C., Duclos, M.J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-09-2017; Elsevier
• Subjects: Animals; BCO1; BCO2; beta Carotene - metabolism; Beta-carotene; beta-Carotene 15,15'-Monooxygenase - metabolism; Cell Differentiation - physiology; Cell Proliferation - physiology; Chicken; Chickens; Life Sciences; Lipid Metabolism; Myoblast; Myoblasts - cytology; Myoblasts - metabolism; Oxidation-Reduction; Retina - metabolism; Retinoic acid; Tretinoin - metabolism; Myoblast; Chicken; BCO2; BCO1; Retinoic acid; Beta-carotene; enzyme; chicken; poulet; myoblast; beta carotène; myoblaste
• ISSN: 0014-4827; 1090-2422
• DOI: 10.1016/j.yexcr.2017.06.011

The enzyme β-carotene oxygenase 1 (BCO1) catalyzes the breakdown of provitamin A, including beta-carotene (BC), into retinal, prior to its oxidation into retinoic acid (RA). Allelic variation at the BCO1 locus results in differential expression of its mRNA and affects carotenoid metabolism specifically in chicken Pectoralis major muscle. In this context, the aim of this study was to evaluate the potential myogenic effect of BC and the underlying mechanisms in chicken myoblasts. BCO1 mRNA was detected in myoblasts derived from chicken satellite cells. Treating these myoblasts with BC led to a significant decrease in BrdU incorporation. This anti-proliferative effect was confirmed by a cell cycle study using flow cytometry. BC also significantly increased the differentiation index, suggesting a positive effect on the commitment of avian myoblasts to myogenic differentiation. Addition of DEAB, a specific inhibitor of RALDH activity, significantly reduced BC anti-proliferative and pro-differentiating effects, suggesting that BC exerted its biological effect on chicken myoblasts through activation of the RA pathway. We also observed that in myoblast showing decreased BCO1 expression consecutive to a natural mutation or to a siRNA treatment, the response to BC was inhibited. Nevertheless, BCO1 siRNA transfection increased expression of BCO2 which inhibited cell proliferation in control and BC treated cells.
Our work identified the BCO1 – RALDH pathway as the major pathway involved in inhibition of proliferation and induction of differentiation by BC (black arrows). For the BCO1 GG genotype, expression of BCMO1 was lower than in AA genotype and BC response was altered (as indicated by red arrows). An alternative RA independent pathway involving BCO2 and the apocarotenoid pathway was suggested in myoblasts transfected with a siRNA against BCO1. Upregulation of BCO2 could also induce a carotenoid independent inhibition of cell proliferation. The dotted arrows indicate incompletely characterized signalling pathways. [Display omitted]
•BCO1 gene is expressed in skeletal myoblasts.•BC reduces proliferation and induces differentiation of skeletal myoblasts.•Inhibition by DEAB shows that BC implies RA production through BCO1 – RALDH pathway.•Reduced BCO1 gene expression blunts the effect of BC on myoblast proliferation.•BCO1 RNA silencing increases BCO2 expression, which could mediate BC effect.