Screening and identification of peritoneal metastasis-related genes of gastric adenocarcinoma using a cDNA microarray

• Published in: Genetics and molecular research Vol. 11; no. 2; pp. 1682 - 1689
• Main Authors: Bai, F H, Wang, N J, Wang, J, Yang, L, Zhang, F M, Yin, F, Liang, J, Wu, K C, Fan, D M
• Format: Journal Article
• Language: English
• Published: Brazil 01-01-2012
• Subjects: Adenocarcinoma - genetics; Humans; Oligonucleotide Array Sequence Analysis - methods; Reverse Transcriptase Polymerase Chain Reaction; Stomach Neoplasms - genetics
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2012.June.25.1

With the aim of identifying peritoneal metastasis-related genes in gastric cancer, we performed a broad analysis of differential gene expression between the parental cell line GC9811 and its highly metastatic peritoneal counterpart, cell line GC9811-P. Two fluorescent cDNA probes, labeled with Cy3 and Cy5 dyes, were prepared from GC9811 and GC9811-P mRNA samples by the reverse transcription method. The two color probes were then mixed and hybridized to a cDNA chip constructed with double-dots from 11,901 human genes; this was scanned at two wavelengths. The experiment was repeated twice. In GC9811-P cells, 218 genes were upregulated and 30 genes were downregulated compared with the parental cell lines. Some selected genes were confirmed by RT-PCR and Western blot; we found that S100A4 and CTNNB1 were upregulated and PTEN was downregulated in GC9811-P cells. Identification of these differentially expressed genes could contribute to disclose the molecular mechanisms involved and provide new targets for therapeutic intervention to avoid peritoneal dissemination of gastric adenocarcinoma.