iPS cell sheets created by a novel magnetite tissue engineering method for reparative angiogenesis

• Published in: Scientific reports Vol. 3; no. 1; p. 1418
• Main Authors: Kito, Tetsutaro, Shibata, Rei, Ishii, Masakazu, Suzuki, Hirohiko, Himeno, Tatsuhito, Kataoka, Yoshiyuki, Yamamura, Yumiko, Yamamoto, Takashi, Nishio, Naomi, Ito, Sachiko, Numaguchi, Yasushi, Tanigawa, Tohru, Yamashita, Jun K., Ouchi, Noriyuki, Honda, Hiroyuki, Isobe, Kenichi, Murohara, Toyoaki
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11-03-2013; Nature Publishing Group
• Subjects: 631/443/592/16; 631/532/489; 639/925/352/1061; 692/699/75/593/1920; Angiogenesis; Animals; Blood flow; Cell Culture Techniques; Cells, Cultured; Extracellular matrix; Extracellular Matrix - metabolism; Fibroblast growth factor 2; Fibroblast Growth Factor 2 - metabolism; Hindlimb - metabolism; Humanities and Social Sciences; Induced Pluripotent Stem Cells - cytology; Induced Pluripotent Stem Cells - metabolism; Induced Pluripotent Stem Cells - transplantation; Ischemia; Ischemia - metabolism; Ischemia - pathology; Ischemia - therapy; Laser-Doppler Flowmetry; Limbs; Liposomes; Liposomes - chemistry; Magnetite; Magnetite Nanoparticles - chemistry; Mice; Mice, Nude; multidisciplinary; Nanoparticles; Neovascularization, Physiologic; Pluripotency; Rodents; Science; Tissue Engineering; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; Vascular endothelial growth factor receptor 2
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/srep01418

Angiogenic cell therapy represents a novel strategy for ischemic diseases, but some patients show poor responses. We investigated the therapeutic potential of an induced pluripotent stem (iPS) cell sheet created by a novel magnetite tissue engineering technology (Mag-TE) for reparative angiogenesis. Mouse iPS cell-derived Flk-1
+
cells were incubated with magnetic nanoparticle-containing liposomes (MCLs). MCL-labeled Flk-1
+
cells were mixed with diluted extracellular matrix (ECM) precursor and a magnet was placed on the reverse side. Magnetized Flk-1
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cells formed multi-layered cell sheets according to magnetic force. Implantation of the Flk-1
+
cell sheet accelerated revascularization of ischemic hindlimbs relative to the contralateral limbs in nude mice as measured by laser Doppler blood flow and capillary density analyses. The Flk-1
+
cell sheet also increased the expressions of VEGF and bFGF in ischemic tissue. iPS cell-derived Flk-1
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cell sheets created by this novel Mag-TE method represent a promising new modality for therapeutic angiogenesis.