Effect of ω-3 Polyunsaturated Fatty Acid on Toll-like Receptors in Patients with Severe Multiple Trauma

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences Vol. 31; no. 4; pp. 504 - 508
• Main Author: 易呈志 白祥军 陈继革 陈驾君 李剑 刘鹏 廖忆刘
• Format: Journal Article
• Language: English
• Published: Heidelberg Huazhong University of Science and Technology 01-08-2011; Department of Traumatic Surgery, Tongji Hospital Tongji Medical College, Huazhong University of Science and Technology, Wuhan430030, China
• Subjects: Adolescent; Adult; Cyclooxygenase-2; ELISA法; Enzyme-linked immunosorbent assay; Fatty Acids, Omega-3 - metabolism; Female; Flow cytometry; Gene expression; Hospitals; Humans; Immunofluorescence; Inflammation; Injuries; Interleukin 2; Interleukin 6; Male; Medicine; Medicine & Public Health; Middle Aged; mRNA; Multiple Trauma - metabolism; NF- Kappa B protein; Nutrition; Parenteral nutrition; Peripheral blood mononuclear cells; Polymerase chain reaction; Polyunsaturated fatty acids; Signal transduction; Surgery; TLR2 protein; TLR4; TLR4 protein; Toll-like receptors; Toll-Like Receptors - metabolism; Toll样受体; Trauma; Tumor necrosis factor- alpha; Young Adult; 外周血单个核细胞; 外周血单核细胞; 多不饱和脂肪酸; 患者; 肿瘤坏死因子; ω-3 polyunsaturated fatty acid; toll-like receptor 4; severe multiple trauma; toll-like receptor 2
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-011-0480-6

This study examined the effects of ω-3 polyunsaturated fatty acid（ω-3PUFA） on the expression of toll-like receptor 2（TLR2）,toll-like receptor 4（TLR4） and some related inflammatory factors in peripheral blood mononuclear cells（PBMCs） of patients with early-stage severe multiple trauma.Thirty-two patients who were admitted to the Department of Traumatic Surgery,Tongji Hospital（Wuhan,China） between May 2010 and November 2010,and diagnosed as having severe multiple trauma with a injury severity score（ISS） no less than 16,were enrolled in the study and divided into two groups at random（n=16 in each）：ω-3PUFA group and control group in which routine parenteral nutrition supplemented with ω-3PUFA or not was administered to the patients in two groups for consecutive 7 days.Peripheral blood from these patients was collected within 2 h of admission（day 0）,and 1,3,5 and 7 days after the nutritional support.PBMCs were isolated and used for detection of the mRNA and protein expression of TLR2 and TLR4 by using real-time PCR and flow cytometry respectively,the levels of NF-κB by quantum dots-based immunofluorescence assay,the levels of TNF-α,IL-2,IL-6 and COX-2 by ELISA,respectively.The results showed that the mRNA and protein expression of TLR2 and TLR4 in PBMCs was significantly lower in ω-3PUFA group than in control group 5 and 7 days after nutrition support（both P0.05）.The levels of TNF-α,IL-2,IL-6 and COX-2 were found to be substantially decreased in PBMCs in ω-3PUFA group as compared with control group at 5th and 7th day（P0.05 for all）.It was concluded that ω-3PUFA can remarkably decrease the expression of TLR2,TLR4 and some related inflammatory factors in NF-κB signaling pathway in PBMCs of patients with severe multiple trauma,which suggests that ω-3PUFA may suppress the excessive inflammatory response meditated by the TLRs/NF-κB signaling pathway.