Phylogenetic relationships of maples (Acer L.; Aceraceae) implied by nuclear ribosomal ITS sequences

• Published in: Journal of Plant Research Vol. 113; no. 1110; pp. 193 - 202
• Main Authors: Suh, Y. (Seoul National Univ., Suwon (Korea R.)), Heo, K, Park, C.W
• Format: Journal Article
• Language: English
• Published: Tokyo The Botanical Society of Japan 01-06-2000; Springer Nature B.V
• Subjects: Acer; ACERACEAE; DNA; Evolutionary biology; GENETIC VARIATION; Genetics; Molecular biology; NUCLEOTIDE SEQUENCE; PHYLOGENY; Ribosomal DNA; RIBOSOMES; TAXONOMY; Trees
• ISSN: 0918-9440; 1618-0860
• DOI: 10.1007/PL00013914

Sequences from internal transcribed spacers (lTS) of nuclear ribosomal DNA were determined to examine phylogenetic relationships in the genus Acer. ITS 1 sequences in twenty-eight species of Acer and a species of Dipteronia in the family Aceraceae ranged from 220 to 242 bp and rrs 2 sequences from 215 to 251 bp. The size of the 5. 8S coding region was 164 bp for all species examined in the family. Phylogenetic analysis of ITS sequences placed a very robust clade of section Palmata at the base of the treo. Threo species of section Parvnilora sensu de Jong (1994). A. spicatum. A. distylum and A. nipponicum, did not form a monophyletic clade. Acer spicatum was separated from the robust clade of A. distylum and A. nipponicum. Molecular tree strongly supports the close relationship among section Platanoidea. Glabra series Arguta, and section Macrantha. The close relationship between sections Pentalphylla and Trnioliata was also strongly suggested in rrs tree. Sections Rubra and Hyptiocarpa appeared to be closely allied with each other. The average rate of nucleotide substitution was estimated as (8. 0±1. 9)×10-11 substitutions per site per year for ITS 1 and (9. 0±1. 6)×10-11 for ITS 2.