Characterization of 22 novel single nucleotide polymorphism markers in steelhead and rainbow trout

Thirty-two individuals representing coastal and inland populations of steelhead and rainbow trout (Oncorhynchus mykiss) were sequenced at 18 expressed sequence tags and nine microsatellite loci to identify single nucleotide polymorphisms. A total of 98 polymorphisms were discovered during the screen...

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Bibliographic Details
Published in:	Molecular ecology resources Vol. 9; no. 1; pp. 318 - 322
Main Authors:	CAMPBELL, NATHAN R, OVERTURF, KEN, NARUM, SHAWN R
Format:	Journal Article
Language:	English
Published:	Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2009 Blackwell Publishing Ltd
Subjects:	5' exonuclease assay Oncorhynchus mykiss rainbow trout SNP steelhead Taqman
Online Access:	Get full text
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Summary:	Thirty-two individuals representing coastal and inland populations of steelhead and rainbow trout (Oncorhynchus mykiss) were sequenced at 18 expressed sequence tags and nine microsatellite loci to identify single nucleotide polymorphisms. A total of 98 polymorphisms were discovered during the screen and 22 were developed into 5' exonuclease assays (Taqman assays). Genotypes from TaqMan assays were compared to sequence data from individuals in the ascertainment panel to confirm proper allele designations. A larger number of samples (n = 192) from six regions were tested with the validated assays. Per-locus FST values ranged from 0.001 to 0.414.
Bibliography:	http://dx.doi.org/10.1111/j.1755-0998.2008.02376.x ArticleID:MEN2376 istex:3EE194655B6741800DF4477005E1799A620FE3B7 ark:/67375/WNG-7ZL3XRWB-S ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	1755-0998 1755-098X 1755-0998
DOI:	10.1111/j.1755-0998.2008.02376.x