Dynamic Microstructure Assembly Driven by Lysinibacillus sp. LF-N1 and Penicillium oxalicum DH-1 Inoculants Corresponds to Composting Performance

• Published in: Microorganisms (Basel) Vol. 10; no. 4; p. 709
• Main Authors: Duan, Haiyan, Fu, Cong, Du, Guilin, Xie, Shiqiu, Liu, Min, Zhang, Baoguo, Shi, Jiping, Sun, Junsong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25-03-2022; MDPI
• Subjects: Acids; Bacteria; Biodegradation; Biomarkers; Cattle manure; Cellulase; Cellulose; Clusters; compost; Composting; Composts; Dairy cattle; Degradation; functional prediction; Fungi; Germination; High temperature; Inoculation; Laboratories; Lignin; Lignocellulose; Lysinibacillus; Manures; Metabolism; Microbial activity; microbial community; Microorganisms; Network analysis; Organic matter; Penicillium; Penicillium oxalicum; Physicochemical properties; Seeds; Wheat straw; United States > US; China; microbial community; Lysinibacillus; Penicillium; compost; functional prediction
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10040709

The effects of Lysinibacillus sp. LF-N1 and Penicillium oxalicum DH-1 inoculants (LFPO group) on compost succession and the microbial dynamic structure of co-composting wheat straw and cow manure composting were investigated. The inoculants contributed to longer thermophilic stages, higher temperatures (62.8 °C) and lower microbial diversity in the LFPO treatment compared to the control group (CK). Moreover, LFPO inoculation increased the germination index and accelerated organic matter and lignocellulose degradation in the compost. Microbial analysis confirmed that the inoculants effectively altered the microbial communities. The predominant biomarkers for bacteria and fungi in inoculated compost were members of Lysinibacillus and Penicillium, respectively. Functional prediction showed greater lignocellulose degradation and less pathogen accumulation in the LFPO group. The cooccurrence network analysis showed that the network structure in LFPO compost was greatly simplified compared to that in CK. Bacterial cluster A was dominated by Lysinibacillus, and fungal cluster B was represented by Penicillium, which were significantly correlated with temperature and lignocellulose degradation, respectively (p < 0.05). These results demonstrated that the LF-N1 and DH-1 inoculants drove the bacterial and fungal assemblies to induce physicochemical property changes during cocomposting.