21 Tesla Fourier Transform Ion Cyclotron Resonance Mass Spectrometer: A National Resource for Ultrahigh Resolution Mass Analysis

• Published in: Journal of the American Society for Mass Spectrometry Vol. 26; no. 9; pp. 1626 - 1632
• Main Authors: Hendrickson, Christopher L., Quinn, John P., Kaiser, Nathan K., Smith, Donald F., Blakney, Greg T., Chen, Tong, Marshall, Alan G., Weisbrod, Chad R., Beu, Steven C.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-09-2015; Springer Nature B.V
• Subjects: Analytical Chemistry; Bioinformatics; Biotechnology; Broadband; Carbonic anhydrase; Chemistry; Chemistry and Materials Science; Cyclotron resonance; Ejection; Electron transfer; Fourier transforms; Ion injection; Ions; Liquid helium; Mass spectrometry; Organic Chemistry; Proteomics; Quadrupoles; Research Article; Resolution; Serum albumin; Superconductivity; Fourier transform mass spectrometry; FT-ICR; FTMS
• ISSN: 1044-0305; 1879-1123
• DOI: 10.1007/s13361-015-1182-2

We describe the design and initial performance of the first 21 tesla Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometer. The 21 tesla magnet is the highest field superconducting magnet ever used for FT-ICR and features high spatial homogeneity, high temporal stability, and negligible liquid helium consumption. The instrument includes a commercial dual linear quadrupole trap front end that features high sensitivity, precise control of trapped ion number, and collisional and electron transfer dissociation. A third linear quadrupole trap offers high ion capacity and ejection efficiency, and rf quadrupole ion injection optics deliver ions to a novel dynamically harmonized ICR cell. Mass resolving power of 150,000 (
m/Δm
50%
) is achieved for bovine serum albumin (66 kDa) for a 0.38 s detection period, and greater than 2,000,000 resolving power is achieved for a 12 s detection period. Externally calibrated broadband mass measurement accuracy is typically less than 150 ppb rms, with resolving power greater than 300,000 at
m/z
400 for a 0.76 s detection period. Combined analysis of electron transfer and collisional dissociation spectra results in 68% sequence coverage for carbonic anhydrase. The instrument is part of the NSF High-Field FT-ICR User Facility and is available free of charge to qualified users.
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