Co-expression of the neuropeptide proctolin and glutamate in the central nervous system, along mechanosensory neurons and leg muscle in Cupiennius salei

• Published in: Cell and tissue research Vol. 382; no. 2; pp. 281 - 292
• Main Authors: Senior, Elizabeth E., Poulin, Hailee E., Dobecki, Madison G., Anair, Bradley M., Fabian-Fine, Ruth
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-11-2020; Springer; Springer Nature B.V
• Subjects: Axons; Biomedical and Life Sciences; Biomedicine; Cellular signal transduction; Central nervous system; Cochlea; Communications equipment; GABA; Ganglia; Glutamate; Hair cells; Human Genetics; Immunohistochemistry; Information processing; Investigations; Leg; Molecular Medicine; Nervous system; Neurons; Neuropeptides; Proctolin; Proteomics; Regular Article; Sense organs; Sensory integration; Sensory neurons; Signal transduction; γ-Aminobutyric acid; Efferent innervation; Co-transmission; Synaptic circuits; Neuropeptide; Central American wandering spider
• ISSN: 0302-766X; 1432-0878
• DOI: 10.1007/s00441-020-03217-6

Similar to hair cells in the mammalian cochlear system, mechanosensory neurons in the Central American wandering spider
Cupiennius salei
are strongly innervated by efferent fibers that originate from neurons whose somata are located in the central nervous system (CNS). In both the mammalian and arachnid systems, efferent fibers have been shown to co-express two or more transmitters; however, our understanding regarding co-transmission and how it affects sensory signal transduction and processing in these systems is only fragmentary. The spider model system is exceptionally suitable for this type of investigation due to the large size and easy accessibility of the sensory and efferent neurons in this system. Thus far, GABA and glutamate have been identified as the main fast-acting transmitters in efferent axons that form synaptic contacts onto sensory neurons in slit sense organs. Ultrastructural investigations suggest an abundance of neuropeptides within these peripheral synapses. In an effort to identify these peptides and conduct functional studies, we have employed immunohistochemistry to investigate whether the neuropeptide proctolin is present in neurons of the leg ganglia and in peripheral leg structures. Here, we demonstrate that ~ 73% of all neurons in the CNS of
C. salei
show proctolin-like immunoreactivity (proc-LIR) including the leg ganglia. We demonstrate that both strongly and weakly labeled neurons can be distinguished. The majority of proc-LIR neurons show weak labeling intensity and ~ 86.2% co-localize with glutamate. In future experiments, we plan to undertake functional studies to investigate the significance of this co-expression, which has yet to be investigated.