AS1411 aptamer-targeted gold nanoclusters effect on the enhancement of radiation therapy efficacy in breast tumor-bearing mice

• Published in: Nanomedicine (London, England) Vol. 13; no. 20; pp. 2563 - 2578
• Main Authors: Ghahremani, Fatemeh, Kefayat, Amirhosein, Shahbazi-Gahrouei, Daryoush, Motaghi, Hasan, Mehrgardi, Masoud A, Haghjooy-Javanmard, Shaghayegh
• Format: Journal Article
• Language: English
• Published: England Future Medicine Ltd 01-10-2018
• Subjects: 4T1; Animals; Aptamers, Nucleotide - administration & dosage; Aptamers, Nucleotide - chemistry; AS1411 aptamer; biocompatibility; Brain cancer; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - pathology; Breast Neoplasms - radiotherapy; breast tumors; cancer; Cancer therapies; Cell Line, Tumor; Drug Delivery Systems; Drug dosages; Female; Flow Cytometry; Fourier transforms; gold nanoclusters; Hemodialysis; Humans; Medical research; Metal Nanoparticles - administration & dosage; Metal Nanoparticles - chemistry; Mice; Nanoparticles; nucleolin; Oligodeoxyribonucleotides - administration & dosage; Oligodeoxyribonucleotides - chemistry; Radiation therapy; Radiation-Sensitizing Agents - administration & dosage; Radiation-Sensitizing Agents - chemistry; radiosensitizer; tumor targeting; Tumors; United States > US; Japan; 4T1; breast tumors; tumor targeting; biocompatibility; gold nanoclusters; radiation therapy; AS1411 aptamer; cancer; radiosensitizer; nucleolin
• ISSN: 1743-5889; 1748-6963
• DOI: 10.2217/nnm-2018-0180

Herein, the AS1411 aptamer-targeted ultrasmall gold nanoclusters (GNCs) were assessed at different aspects as a radiosensitizer.
AS1411 aptamer-conjugated gold nanoclusters (Apt-GNCs) efficacy was evaluated at cancer cells targeting, radiosensitizing effect, tumor targeting, and biocompatibility in breast tumor-bearing mice.
Flow cytometry and fluorescence microscopy exhibited more cellular uptake for Apt-GNCs in comparison with GNCs. In addition, inductively coupled plasma optical emission spectrometry results demonstrated its effective tumor targeting as the tumors' gold content for GNCs and Apt-GNCs were 8.53 and 15.33 μg/g, respectively. Apt-GNCs significantly enhanced radiotherapy efficacy as mean tumors' volume decreased about 39% and 9 days increase in the mice survival was observed. Both GNCs and Apt-GNCs were biocompatible.
The Apt-GNCs is a novel and efficient radiosensitizer.