The Saccharomyces cerevisiae mevalonate diphosphate decarboxylase (Erg19p) forms homodimers in vivo, and a single substitution in a structurally conserved region impairs dimerization

• Published in: Current microbiology Vol. 38; no. 5; pp. 290 - 294
• Main Authors: CORDIER, H, LACOMBE, C, KARST, F, BERGES, T
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01-05-1999; Springer Nature B.V
• Subjects: Adenine; Amino Acid Sequence; Amino Acid Substitution; Amino acids; Biological and medical sciences; Carboxy-Lyases - genetics; Carboxy-Lyases - metabolism; Culture Media - chemistry; Dimerization; Fundamental and applied biological sciences. Psychology; Genes, Fungal; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Histidine; Microbiology; Molecular Sequence Data; Mutation; Mycology; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Sequence Homology, Amino Acid; Yeast; Yeasts; Yeast; Enzyme; Lyases; Secondary structure; Diphosphomevalonate decarboxylase; Fungi; Carbon-carbon lyases; Gene; Substitution; Carboxy-lyases; Ascomycetes; Property structure relationship; Mutation; Dimerization; Saccharomyces cerevisiae; Thallophyta; Structural analysis
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/pl00006804

The wild-type ERG19 gene of the yeast Saccharomyces cerevisiae encoding mevalonate diphosphate decarboxylase (MVD) and the mutated recessive erg19-34 allele leading to a decrease of sterol production and to a thermosensitive phenotype have been characterized [2]. The mutated erg19-34 allele bears a single amino acid leucine 79-to-proline (L79P) substitution. It was shown that this mutation does not affect the level of production of the enzyme. We performed a two-hybrid assay to show that the yeast Saccharomyces cerevisiae MVD forms homodimers in vivo and that the single point mutation drastically impairs the oligomerization of the protein, thereby explaining the deficiency of MVD activity observed in the temperature-sensitive strain.