Profiling of Brevibacillus borstelensis transcriptome exposed to high temperature shock

• Published in: Genomics (San Diego, Calif.) Vol. 107; no. 1; pp. 33 - 39
• Main Authors: Tripathy, S., Padhi, S.K., Sen, R., Mohanty, S., Samanta, M., Maiti, N.K.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-01-2016
• Subjects: B. borstelensis; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Brevibacillus; Brevibacillus - genetics; Brevibacillus - metabolism; Gene expression profile; Gene Expression Regulation, Bacterial; Heat shock; Heat-Shock Response; HSP90 Heat-Shock Proteins - genetics; HSP90 Heat-Shock Proteins - metabolism; Membrane Transport Proteins - genetics; Membrane Transport Proteins - metabolism; RT-PCR; Transcription Factors - genetics; Transcription Factors - metabolism; Transcriptome; C; DGE; NR; HSP; GO; mRNA; EIP; B. borstelensis; GIP; Gene expression profile; RT-PCR; KEGG; cDNA; BLAST; Heat shock
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2015.11.005

To understand the molecular mechanisms underlying the ability of the bacteria to survive at high temperature, gene expression profile of Brevibacillusborstelensis at 55°C during 5 and 10min heat shock period was carried out by high-throughput sequencing technology. A total of 2555 non-redundant transcripts were annotated. A total of 575 genes at 5min and 400 genes at 10min exhibited significant differential expression in response to temperature upshift from 50 to 55°C. Genes up-regulated under heat shock were associated with metabolism (mtnE), membrane transport, signal transduction, transcriptional regulation (ycxD, codY) and folding and sorting (hsp90). A larger number of genes encoding hypothetical proteins were identified. RT-PCR experimental results carried out on genes expressed under heat shock were found to be consistent with transcriptome data. The results enhance our understanding of adaptation strategy of thermophilic bacteria thereby providing a strong background for in depth research in thermophiles.
•‘Amino acid metabolism and transport’ represented the highest COG category.•Most of the genes were mapped to ‘biological processes’ as per GO classification.•Majority of the DEGs mapped to KEGG pathways were under ‘metabolism’.•Stress response and metabolic proteins were induced.•Greater number of hypothetical proteins were up-regulated.