Effect of potassium intake on cadmium transporters and root cell wall biosynthesis in sweet potato

• Published in: Ecotoxicology and environmental safety Vol. 250; p. 114501
• Main Authors: Huang, Baifei, Liao, Qiong, Fu, Huiling, Ye, Ziyi, Mao, Yixiao, Luo, Jiemei, Wang, Yating, Yuan, Haiwei, Xin, Junliang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 15-01-2023; Elsevier
• Subjects: Bioaccumulation; Biosynthesis; Cadmium (Cd); Cadmium - metabolism; Cadmium - toxicity; Cell Wall - metabolism; Ipomoea batatas - chemistry; Macro-element; Plant Roots - metabolism; Potassium - metabolism; RNA-sequencing; Sweet potato; Cd; CaM; NRAMP; NRT; SULTR; CHX; DW; Bioaccumulation; KEGG; CYP; SKOR; POD; CAD; FCLY; RBOH; SUS; ROS; HCT; CAX; SAM; ABA; WAK; Cadmium (Cd); RCW; IRT; Biosynthesis; K; INT; MRS; TPP; Sweet potato; FTIR; PHO; TPS; UDP-G; LHT; Macro-element; GSH; ACC; DEG; GO; PME; COPT; RNA-sequencing; ACS; HAK; YSL; F5H; CLC; TEM; FRK
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2023.114501

Large areas of farmland soil in southern China are deficient in potassium (K) and are contaminated with cadmium (Cd). Previously, we suggested that the K supplementation could reduce Cd accumulation in sweet potatoes (Ipomoea batatas (L.) Lam). In the present study, we investigated the underlying physiological and molecular mechanisms. A hydroponic experiment with different K and Cd treatments was performed to compare the transcriptome profile and the cell wall structure in the roots of sweet potato using RNA sequencing, Fourier transform infrared spectroscopy (FTIR) and transmission electron microscopy (TEM). The results showed that K supply inhibits the expressions of IRT1 and YSL3, which are responsible for root Cd uptake under Cd exposure. Furthermore, the expressions of COPT5 and Nramp3 were downregulated by K, which increased Cd retention in the root vacuoles. The upregulation of POD, CAD, INT1 and SUS by K contributed to lignin and cellulose biosynthesis and thickening of root xylem cell wall, which further reduced Cd translocation to the shoot. In addition, K affected the expressions of LHT, ACS, TPS and TPP associated with the production of ethylene and trehalose, which involved in plant resistance to Cd toxicity. In general, K application could decrease the uptake and translocation of Cd in sweet potatoes by regulating the expression of genes associated with Cd transporters and root cell wall components.
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•Potassium (K) supply decreased cadmium (Cd) accumulation in sweet potato plants.•K downregulated the expression of YSL3 and IRT1 responsible for root Cd uptake.•K increased Cd retention in root vacuoles by downregulating COPT5 and Nramp3.•K upregulated the expression of genes related to the biosynthesis of cell walls.