Characterization of graphite electrodes modified with laccase from Trametes versicolor and their use for bioelectrochemical monitoring of phenolic compounds in flow injection analysis

• Published in: Analytica chimica acta Vol. 487; no. 1; pp. 3 - 14
• Main Authors: Haghighi, B., Gorton, L., Ruzgas, T., Jönsson, L.J.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01-07-2003; Elsevier
• Subjects: Amperometry; Analytical Chemistry; Analytisk kemi; Biological and medical sciences; Biosensors; Biotechnology; Chemical Sciences; Chemistry; Coriolus hirsutus; Electrochemical methods; Exact sciences and technology; Flow injection; Fundamental and applied biological sciences. Psychology; Kemi; Laccase; Methods. Procedures. Technologies; Natural Sciences; Naturvetenskap; Phanerochaete chrysosporium; Phenols; Trametes versicolor; Various methods and equipments; Amperometry; Phenols; Biosensors; Flow injection; Laccase; Electrode adsorption; Enzyme; Coriolus versicolor; Basidiomycetes; Pyrocatechol; Fungi; Reproducibility; Biosensor; Standard deviation; Oxidoreductases; Thallophyta
• ISSN: 0003-2670; 1873-4324; 1873-4324
• DOI: 10.1016/S0003-2670(03)00077-1

Spectrographic graphite electrodes were modified through adsorption with laccase from
Trametes versicolor. The laccase-modified graphite electrode was used as the working electrode in an amperometric flow-through cell for monitoring phenolic compounds in a single line flow injection system. The experimental conditions for bioelectrochemical determination of catechol were studied and optimized. The relative standard deviation of the biosensor for catechol (10
μM,
n=12) was 1.0% and the reproducibility for six laccase-modified graphite electrodes, prepared and used different days was about 11%. The optimal conditions for the biosensor operation were: 0.1
M citrate buffer solution ( at pH 5.0), flow rate of 0.51
ml
min
−1 and a working potential of −50
mV versus Ag|AgCl. At these conditions the responses of the biosensor for various phenolic compounds were recorded and the sensor characteristics were calculated and compared with those known for biosensors based on laccase from
Coriolus hirsutus, cellobiose dehydrogenase (CDH) from
Phanerochaete chrysosporium and horseradish peroxidase (HRP).