Association of a protein phosphatase 1 activity with the human factor C1 (HCF) complex

• Published in: Nucleic acids research Vol. 28; no. 3; pp. 678 - 686
• Main Authors: Ajuh, P M, Browne, G J, Hawkes, N A, Cohen, P T, Roberts, S G, Lamond, A I
• Format: Journal Article
• Language: English
• Published: England Oxford Publishing Limited (England) 01-02-2000; Oxford University Press
• Subjects: Apoptosis Regulatory Proteins; Base Sequence; Binding, Competitive; Blotting, Western; C1 factor; Carrier Proteins - chemistry; Carrier Proteins - metabolism; Catalytic Domain; Cell Extracts; Cloning, Molecular; Consensus Sequence - genetics; Digoxigenin; Gene Library; HeLa Cells; Herpes simplex virus; Herpes Simplex Virus Protein Vmw65 - genetics; Herpes Simplex Virus Protein Vmw65 - metabolism; Host Cell Factor C1; Humans; immediate early genes; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Okadaic Acid - pharmacology; phosphoprotein phosphatase 1; Phosphoprotein Phosphatases - antagonists & inhibitors; Phosphoprotein Phosphatases - chemistry; Phosphoprotein Phosphatases - metabolism; Precipitin Tests; Protein Binding; Protein Phosphatase 1; Proteins - chemistry; Proteins - genetics; Proteins - metabolism; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Transcription Factors; VP16 protein
• ISSN: 1362-4962; 0305-1048; 1362-4962
• DOI: 10.1093/nar/28.3.678

We have screened a human cDNA expression library with a digoxygenin-labelled protein phosphatase 1 (PP1) probe to identify novel PP1 interacting proteins. Eleven cDNA clones were isolated, which included genes encoding two previously characterised and six novel PP1 binding proteins. Three of the cDNAs encoded a protein called host cell factor (HCF), which is an essential component of the cellular complex required for the transcription of the herpes simplex virus (HSV) immediate-early (IE) genes. We demonstrate that HCF and PP1 exist as a complex in nuclear extracts and that this complex is distinct from the form of HCF that associates with HSV VP16. The data suggest novel roles for HCF and PP1, which may be relevant to their functions in transcription and cell cycle progression.