Chromobacterium violaceum delivers violacein, a hydrophobic antibiotic, to other microbes in membrane vesicles

• Published in: Environmental microbiology Vol. 22; no. 2; pp. 705 - 713
• Main Authors: Choi, Seong Yeol, Lim, Sungbin, Cho, Gayoung, Kwon, Jisoo, Mun, Wonsik, Im, Hansol, Mitchell, Robert J.
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01-02-2020; Wiley Subscription Services, Inc
• Subjects: Antibiotics; Chromobacterium violaceum; Extracellular; Hydrophobicity; Membrane vesicles; Membranes; Microorganisms; Mutants; Protein folding; Transport; Vesicles; Viability; Violacein
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.14888

Summary
This study describes Chromobacterium violaceum's use of extracellular membrane vesicles (MVs) to both solubilize and transport violacein to other microorganisms. Violacein is a hydrophobic bisindole with known antibiotic activities against other microorganisms. Characterization of the MVs found they carried more violacein than protein (1.37 ± 0.19‐fold), suggesting they may act as a reservoir for this compound. However, MVs are not produced in response to violacein – a ΔvioA isogenic mutant, which is incapable of making violacein, actually produced significantly more MVs (3.2‐fold) than the wild‐type strain. Although violacein is insoluble in water (Log Poctanol:water = 3.34), 79.5% remained in the aqueous phase when it was present within the C. violaceum MVs, an increase in solubility of 1740‐fold. Moreover, tests with a strain of Staphylococcus aureus showed MV‐associated violacein is bactericidal, with 3.1 mg/l killing 90% of S. aureus in 6 h. Tests with the ΔvioA MVs found no loss in the S. aureus viability, even when its MVs were added at much higher concentrations, demonstrating violacein is the active component within the wild‐type MVs. In conclusion, our study clearly demonstrates C. violaceum produces MVs and uses them as vehicles to solubilize violacein and transport this hydrophobic antibiotic to other microbes.