Genotoxicity evaluation of five different dentin bonding agents by chromosomal aberration analysis

• Published in: Journal of oral rehabilitation Vol. 33; no. 6; pp. 462 - 471
• Main Authors: PRICA, D., GALIĆ, N., ŽELJEŽIĆ, D., PRICA, A.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-06-2006
• Subjects: adhesives; Case-Control Studies; Chi-Square Distribution; Chromatids - ultrastructure; chromosomal aberrations; Chromosome Aberrations; dentin bonding agents; Dentin Permeability; Dentin-Bonding Agents - toxicity; genotoxicity; Humans; Lymphocytes - drug effects; Lymphocytes - ultrastructure; Mutagenicity Tests; Mutagens
• ISSN: 0305-182X; 1365-2842
• DOI: 10.1111/j.1365-2842.2006.01606.x

summary  Dentin bonding agents became unavoidable in today's aesthetic restorative dentistry. Nevertheless, more and more evidences on their possible cytotoxity and/or genotoxicity emerge. Still, only limited number of studies has been published on that issue. In our work we evaluated possible genotoxicity of five different adhesives: AdperTR Single Bond, AdperTR Single Bond 2 with nanofiller, Excite®, OptiBond® Solo PlusTR and PromptTR L‐pop. Genotoxicity assessment was carried out on human lymphocytes in vitro, using chromosomal aberration analysis. Polymerized adhesives were tested at three different dilutions of the 0·5 g mL−1 eluate stock (2·5 × 1:106, 1:106 and 1:105) after 1 h, 24 h and 5 days of elution. Slight but significant increase in the number of chromatid breaks was observed after 24‐h elution period, for adhesives AdperTR Single Bond 2, Excite®, and OptiBond® Solo PlusTR at dilutions of 1:106 and 1:105, and for other two only at dilution of 1:105. First three adhesives also appeared to be slightly genotoxic after 1 h of elution but only at 1:105. As a bonding agent remains in close contact with living dental tissue over a long period of time, information on their possible genotoxicity and carcinogenicity should be more clearly clarified in the near future.