Micropropagation of self-rooting juvenile clones by secondary somatic embryogenesis in Hevea brasiliensis

• Published in: Plant breeding Vol. 129; no. 2; pp. 202 - 207
• Main Authors: Hua, Y.W, Huang, T.D, Huang, H.S
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01-04-2010; Blackwell Publishing Ltd; Blackwell
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; embryo multiplication; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Haploidy, in vitro culture applications, somatic hybrids; Hevea brasiliensis; Plant breeding: fundamental aspects and methodology; secondary somatic embryogenesis; self-rooting juvenile clone; Embryonic development; Hevea brasiliensis; Laticiferous plant; Tissue culture; Micropropagation; Somatic embryo; secondary somatic embryogenesis; self-rooting juvenile clone; Dicotyledones; Angiospermae; Genetic improvement; Euphorbiaceae; Hardwood forest tree; Spermatophyta; Rooting; embryo multiplication; Clone
• ISSN: 0179-9541; 1439-0523
• DOI: 10.1111/j.1439-0523.2009.01663.x

With 2 figures and 5 tables Micropropagation of self-rooting juvenile clones in Hevea brasiliensis was established for two clones CATAS 7-33-97 and CATAS 88-13 through the following three steps: induction of primary embryos, embryo multiplication by secondary somatic embryogenesis in three successive cycles from a single culture of primary embryo and plant regeneration. The embryo multiplication coefficients of the two clones increased in the first cycle and reached the maximum in the second and the third cycle at the same rate. Significant effects of origins of embryo fragments and calcium on secondary embryogenesis were detected, the highest ratios of the regenerated embryos to primary embryos appeared, when embryo fragments close to the base of embryos were used and incubated in Murashige and Skoog (MS)-based callogenesis medium with 6.0 m m CaCl₂ for CATAS 88-13 and 9.0 m m CaCl₂ for CATAS 7-33-97. The highest rates of plant conversion were produced on MS-based plant regeneration medium with 4.5 and 9.0 μ m 2,4-D for CATAS 7-33-97 (85.0%) and 13.5 μ m for CATAS 88-13 (75.0%), being higher than other reports (60%). Finally, the application of this system was discussed.