Modulation of human neutrophil oxidative metabolism and degranulation by extract of Tamarindus indica L. fruit pulp

• Published in: Food and chemical toxicology Vol. 47; no. 1; pp. 163 - 170
• Main Authors: Paula, Fabiana S., Kabeya, Luciana M., Kanashiro, Alexandre, de Figueiredo, Andréa S.G., Azzolini, Ana Elisa C.S., Uyemura, Sérgio A., Lucisano-Valim, Yara Maria
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 2009; Elsevier
• Subjects: antioxidant activity; antioxidants; Antioxidants - chemistry; Antioxidants - pharmacology; Biological and medical sciences; Cell Degranulation - drug effects; Cells, Cultured; chemical constituents of plants; Chemiluminescence; Elastase; Female; free radical scavengers; Fruit - chemistry; fruit composition; fruit extracts; fruit pulp; fruit trees; fruits (food); General pharmacology; Humans; indigenous species; Luminescent Measurements; Male; Medical sciences; NADPH Oxidases - antagonists & inhibitors; Neutrophils; Neutrophils - drug effects; Neutrophils - metabolism; Oriental traditional medicine; Pancreatic Elastase - antagonists & inhibitors; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; Phorbol Esters; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant poisons toxicology; polyphenols; Reactive oxygen species; Reactive Oxygen Species - metabolism; Tamarindus - chemistry; Tamarindus indica; Toxicology; Asia; NADPH; IC 50; Reactive oxygen species; p-NA; fMLP; DMSO; AUC; LDH; ANOVA; LDL; Elastase; CB; ExT; SAAVNA; PMA; LucCL; Neutrophils; LumCL; CL; Chemiluminescence; OZ; c.p.m; ROS; SEM; HBSS; Tamarindus indica; Oxidative stress; Granulocyte; Extract; Medicinal plant; Pulp; Dicotyledones; Modulation; Angiospermae; Fruit tree; Human; Fruit; Nutrition; Metabolism; Feeding; Leguminosae; Diet; Plant origin; Degranulation; Spermatophyta; Neutrophil
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2008.10.023

The tamarind (
Tamarindus indica L
.) is indigenous to Asian countries and widely cultivated in the American continents. The tamarind fruit pulp extract (ExT), traditionally used in spices, food components and juices, is rich in polyphenols that have demonstrated anti-atherosclerotic, antioxidant and immunomodulatory activities. This study evaluated the modulator effect of a crude hydroalcoholic ExT on some peripheral human neutrophil functions. The neutrophil reactive oxygen species generation, triggered by opsonized zymosan (OZ),
n-formyl-methionyl-leucyl-phenylalanine (fMLP) or phorbol myristate acetate (PMA), and assessed by luminol- and lucigenin-enhanced chemiluminescence (LumCL and LucCL, respectively), was inhibited by ExT in a concentration-dependent manner. ExT was a more effective inhibitor of the PMA-stimulated neutrophil function [IC
50 (in μg/10
6cells)
=
115.7
±
9.7 (LumCL) and 174.5
±
25.9 (LucCL)], than the OZ- [IC
50
=
248.5
±
23.1 (LumCL) and 324.1
±
34.6 (LucCL)] or fMLP-stimulated cells [IC
50
=
178.5
±
12.2 (LumCL)]. The ExT also inhibited neutrophil NADPH oxidase activity (evaluated by O
2 consumption), degranulation and elastase activity (evaluated by spectrophotometric methods) at concentrations higher than 200
μg/10
6
cells, without being toxic to the cells, under the conditions assessed. Together, these results indicate the potential of ExT as a source of compounds that can modulate the neutrophil-mediated inflammatory diseases.