Extracts of dietary plants are efficient modulators of nuclear factor kappa B

• Published in: Food and chemical toxicology Vol. 46; no. 4; pp. 1288 - 1297
• Main Authors: Paur, I., Austenaa, L.M., Blomhoff, R.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01-04-2008; New York, NY Elsevier Science
• Subjects: Biological and medical sciences; Cell Line; cell lines; Diet; DNA; DNA - metabolism; DNA binding; Drug Evaluation, Preclinical; Genes, Reporter - genetics; Humans; Inhibitor; Lipopolysaccharides - pharmacology; luciferase; Luciferases - genetics; Medical sciences; monocytes; Monocytes - drug effects; Monocytes - metabolism; NF-kappa B - drug effects; NF-κB; nuclear factor kappa B; Oxidation-Reduction; Phytochemicals; plant extracts; Plant Extracts - pharmacology; Plant poisons toxicology; plant-based foods; Plants, Edible - chemistry; protective effect; Reference Standards; Toxicology; transcription (genetics); Transcription Factor RelA - biosynthesis; Transcription Factor RelA - genetics; transcription factors; Transfection; U937 Cells; IL; DMSO; EGCG; TRADD; IKK; TNF; LPS; TNFR1; IκB; IRAK; NAC; NF-κB; Phytochemicals; Diet; TRAF6; FRAP; Inhibitor; TLR4; MYD88; Modulator; Nutrition; Oral administration; NF-KB; Extract; Feeding; Signal transduction; Plant; Plant origin; Transcription factor NFκB
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2007.09.103

Convincing evidence suggest that a plant-based diet is associated with a reduced risk of several chronic diseases, but the mechanisms for this association is not fully elucidated. The transcription factor nuclear factor kappa B (NF-κB) plays a critical role in cellular stress-, immune- and inflammatory responses. Also, NF-κB is identified as a promising therapeutic target both in cancer and chronic inflammation. We used monocytes stably transfected with a NF-κB-luciferase reporter construct in a screening of plant extracts for NF-κB modulators. Our aim was to identify dietary components which could induce basal NF-κB activity to produce a preconditioning effect, or inhibit induction of disease related NF-κB activity. When screening 34 dietary plants for their ability to induce basal NF-κB activity or inhibit lipopolysaccharide induced NF-κB activity we observed that 23 dietary plant extracts induced basal NF-κB activity, while 15 extracts attenuate induced NF-κB activation. These results indicate that dietary plants contain compounds that efficiently modulate NF-κB activity. We suggest dietary modulation of NF-κB may contribute to the observed beneficial effects of dietary plants on the risk of chronic diseases.