The ribosomal ribonucleic acid of Agrobacterium tumefaciens

The 23S rRNA of Agrobacterium tumefaciens contains at least two nicks which result in the formation of RNA components with mol.wts. of 0.52 X 10(6) and 0.48 X 10(6). Thus under the usual conditions of extraction and analysis, no 23S rRNA was recovered from the bacterium. The experiments show that 23...

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Bibliographic Details
Published in:	Biochemical journal Vol. 149; no. 1; pp. 17 - 22
Main Authors:	Schuch, W, Loening, U E
Format:	Journal Article
Language:	English
Published:	England 01-07-1975
Subjects:	Electrophoresis, Polyacrylamide Gel Magnesium Molecular Weight Rhizobium - analysis Ribosomes - analysis RNA, Ribosomal - analysis
Online Access:	Get full text
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Summary:	The 23S rRNA of Agrobacterium tumefaciens contains at least two nicks which result in the formation of RNA components with mol.wts. of 0.52 X 10(6) and 0.48 X 10(6). Thus under the usual conditions of extraction and analysis, no 23S rRNA was recovered from the bacterium. The experiments show that 23S rRNA is synthesized as a continuous chain, in which one or two nicks are formed almost immediately near the ends of the molecule and an additional nick in the middle at a later time.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0264-6021 1470-8728
DOI:	10.1042/bj1490017