Further analysis on the structural proteins of infectious pancreatic necrosis virus

The structural proteins of infectious pancreatic necrosis virus (IPNV) have been analyzed. Two-dimensional gel electrophoresis showed that IPNV proteins are slightly acidic with apparent pIs ranging from 5.8 to 6.6. To identify the IPNV surface-located proteins, purified virus was labelled either wi...

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Bibliographic Details
Published in:	Virus research Vol. 15; no. 1; p. 85
Main Authors:	Estay, A, Farias, G, Soler, M, Kuznar, J
Format:	Journal Article
Language:	English
Published:	Netherlands 01-01-1990
Subjects:	Animals Capsid - analysis Capsid Proteins Concanavalin A - analogs & derivatives Fluorescein-5-isothiocyanate - analogs & derivatives Fluoresceins Glycosylation Isoelectric Point Necrosis Pancreatic Diseases - microbiology Pancreatic Diseases - pathology Salmonidae Viral Structural Proteins - analysis Viruses - analysis
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Summary:	The structural proteins of infectious pancreatic necrosis virus (IPNV) have been analyzed. Two-dimensional gel electrophoresis showed that IPNV proteins are slightly acidic with apparent pIs ranging from 5.8 to 6.6. To identify the IPNV surface-located proteins, purified virus was labelled either with fluorescein isothiocyanate (FITC) or with Na 125I. After analysis by SDS-PAGE, only the major viral protein, VP2, was labelled by either procedure. The accessibility of VP2 to these reagents suggests that this protein is externally located. In addition, using Concanavalin A conjugated with FITC and IPNV labelling with 3H-mannose, evidence is present that VP2 contains carbohydrate residues.
ISSN:	0168-1702
DOI:	10.1016/0168-1702(90)90015-4