Biochemical identification of B-F and B-G allelic variants of the chicken major histocompatibility complex

• Published in: Animal genetics Vol. 22; no. 4; p. 323
• Main Authors: Hepkema, B G, van der Poel, A, Grosfeld-Stulemeyer, M C, Hensen, E J
• Format: Journal Article
• Language: English
• Published: England 01-01-1991
• Subjects: Alleles; Animals; Chickens - immunology; Cross Reactions; Erythrocytes - chemistry; Erythrocytes - immunology; Genetic Variation - genetics; Histocompatibility Antigens - chemistry; Histocompatibility Antigens - genetics; Immunoblotting; Isoelectric Focusing
• ISSN: 0268-9146
• DOI: 10.1111/j.1365-2052.1991.tb00686.x

Biochemical methods were used to analyse B-F and B-G antigens of the chicken major histocompatibility complex (MHC). In a panel of 12 inbred or partially inbred chicken lines the MHC haplotypes, originally defined by serological and histogenetical methods, were compared. Using monoclonal 18-6G2, allele-specific B-G patterns were obtained by immunoblotting. Comparison of B-G12 and B-G2 revealed a shared banding pattern, but additional products were detected for B-G12. The B-F products of B2 and B12 had identical IEF patterns. The identical B-F products and partially shared B-G products might explain the serological cross-reaction between these haplotypes. In addition, the IEF pattern of B-F21 appeared similar to B-F2 and B-F12, but the partial proteolysis map showed a clear difference. Although two B-F bands could be detected per haplotype, no evidence for the expression of more than one B-F locus was found. The biochemical methods enabled a precise definition of expressed MHC products and can be a useful tool for the identification of B-alleles in other chicken lines or outbred chickens for their MHC antigens.