Enhanced Saccharification of Rice Straw Using Hypochlorite-hydrogen Peroxide

• Published in: Biotechnology and bioprocess engineering Vol. 16; no. 2; pp. 273 - 281
• Main Authors: Kang, H.K., Chonnam National University, Gwangju, Republic of Korea, Kim, N.H., University of Southern California, CA, USA, Kim, G.H., University of California San Diego, CA, USA, Seo, E.S., Chonnam National University, Gwangju, Republic of Korea, Ryu, H.J., Chonnam National University, Gwangju, Republic of Korea, Yun, S.I., Chonnam National University, Gwangju, Republic of Korea, Choi, H.C., Chonnam National University, Gwangju, Republic of Korea, Day, Donal F., Louisiana State University, LA, USA, Kim, J.H., Chonnam National University, Gwangju, Republic of Korea, Cho, D.L., Chonnam National University, Gwangju, Republic of Korea, Kim, D.M., Chonnam National University, Gwangju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Heidelberg The Korean Society for Biotechnology and Bioengineering 01-04-2011; Springer Nature B.V
• Subjects: Alternative energy sources; Biomass; Biotechnology; Cellulose; Chemistry; Chemistry and Materials Science; Endoglucanase; Energy; ETANOL; ETHANOL; Experiments; Fermentation; Glucose; Hydrogen peroxide; Hydrolysates; hypochlorite-hydrogen peroxide; Industrial and Production Engineering; Lignin; Lignocellulose; lignocelluloses; methylcellulose; Oryza sativa; PICHIA STIPITIS; pretreatment; Raw materials; Research Paper; Rice straw; Straw; Studies; Variance analysis; hypochlorite-hydrogen peroxide; lignocelluloses; ethanol; pretreatment
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-010-0262-1

Rice straw is a lignocellulosic biomass, and has been recognized as a renewable organic substance and alternative energy source. In this study, rice straw was pretreated with hypochlorite-hydrogen peroxide (Ox-B) solution. The optimal pretreatment conditions were determined via response surface methodology, and the pretreated rice straw was hydrolyzed with exo-glucanase, endoglucanase, hemicellulase, and β-glucosidase Accellerase 1000∨TM (endo-glucanase equivalent activity of 1,250 carboxy methyl cellulose (CMC) U/g of rice straw pretreated for 24 h). The optimal conditions were as follows: 60 min pretreatment using Ox-B solution containing 0.6% hypochlorite and 25% hydrogen peroxide for 1 g of rice straw in a total reaction volume of 240 mL. Under these conditions, 406.8 mg of D-glucose and 224.0 mg of D-xylose were obtained from 1 g of rice straw. The fermentation of enzymatic hydrolysates containing 8.14 g/L D-glucose and 4.49 g/L D-xylose with Pichia stipitis generated 3.65 g/L of ethanol with a corresponding yield of 0.37 g/g. The maximum possible ethanol conversion rate is 72.54%.