Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

• Published in: Nature (London) Vol. 309; no. 5967; pp. 418 - 425
• Main Authors: Ullrich, A, Coussens, L, Hayflick, J. S, Dull, T. J, Gray, A, Tam, A. W, Lee, J, Yarden, Y, Libermann, T. A, Schlessinger, J, Downward, J, Mayes, E. L. V, Whittle, N, Waterfield, M. D, Seeburg, P. H
• Format: Journal Article
• Language: English
• Published: London Nature Publishing 01-05-1984
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Carcinoma, Squamous Cell; Cell Line; DNA - metabolism; Epidermal Growth Factor - metabolism; ErbB Receptors; Fundamental and applied biological sciences. Psychology; Gene Amplification; Gene expression; Genes; Humans; Molecular and cellular biology; Molecular genetics; Nucleic Acid Hybridization; Peptide Fragments - analysis; Receptors, Cell Surface - genetics; Receptors, Cell Surface - metabolism; RNA, Messenger - genetics; Homology; Primary structure; Binding site; Gene expression; Proteins; Gene amplification; Membrane receptor; Epidermal growth factor; Cell line; Complementary DNA; Gene; Gene rearrangement; Tumor; Tumor cell
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/309418a0

The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted v-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells, generating a truncated 2.8-kilobase mRNA which encodes only the extracellular EGF binding domain.