Molecular systematics of medusae in the genus Craspedacusta (Cnidaria: Hydrozoa: Limnomedusae) in China with the reference to the identity of species

• Published in: Journal of plankton research Vol. 31; no. 5; pp. 563 - 570
• Main Authors: Zhang, Li Q., Wang, Gui T., Yao, Wei J., Li, Wen X., Gao, Qian
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-05-2009; Oxford Publishing Limited (England)
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Aquatic invertebrates; Biological and medical sciences; Centrochthonius sichuanensis; Cnidaria; Cnidaria. Ctenaria; Craspedacusta; Craspedacusta kiatingi; Craspedacusta sinensis; Divergence; DNA; Fundamental and applied biological sciences. Psychology; General aspects; Hydrozoa; Interspecific; Invertebrates; Limnomedusae; Marine; Marine invertebrates; Medusae; Nesting; Nucleotide sequence; Phylogenetics; Phylogeny; Samples; Sequencing; Species; Synecology; Asia; China; INW, China, People's Rep; Coelenterata; Hydrozoa; Molecular systematics; Plankton; Invertebrata; Cnidaria
• ISSN: 0142-7873; 1464-3774
• DOI: 10.1093/plankt/fbp005

The objective of this study was to illustrate the phylogenetic relationship of the species in the genus Craspedacusta in China. The medusae samples were collected at 28 localities in China representing seven described species with their entire ITS region (the contiguous sequences of ITS-1, 5.8S and ITS-2 rDNA) rDNA sequences cloned. Among the 28 samples, the range of sequence variation in the complete ITS and 5.8S region was between 0 and 36.2%. Three main clades were revealed by both maximum likelihood and neighbour-joining trees, with sequence difference of 0–0.9, 0–3.7 and 0.1–1.5% in the three clades. The nesting of C. xinyangensis representatives within C. sowerbii, C. brevinema within C. sinensis and C. sichuanensis within C. kiatingi is strongly supported, with interspecific sequence divergence of 0–0.9, 0.1–1.4 and 0.0–0.4%, respectively. Thus, it is suggested that C. xinyangensis should be the synonym of C. sowerbii, C. sichuanensis the synonym of C. kiatingi and C. brevinema the synonym of C. sinensis. However, the taxonomic status of C. ziguiensis is still uncertain. According to the tree topology, C. kiatingi was closer to C. sowerbii than to C. sinensis. Craspedacusta sinensis was the most genetically distinct from distance matrix values, and located at the base of the phylogenetic trees, so it can be speculated that the C. sinensis may be the ancestral form in the genus Craspedacusta.