Role of BMPs in controlling the spatial and temporal origin of GFAP astrocytes in the embryonic spinal cord

• Published in: Developmental biology Vol. 344; no. 2; pp. 611 - 620
• Main Authors: Agius, Eric, Decker, Yann, Soukkarieh, Chadi, Soula, Cathy, Cochard, Philippe
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15-08-2010
• Subjects: Animals; Astrocyte; Astrocytes - metabolism; BMP; Bone Morphogenetic Proteins - metabolism; Bone Morphogenetic Proteins - physiology; Cell Differentiation; Chick Embryo; Development; Embryo, Nonmammalian; Glial Fibrillary Acidic Protein - metabolism; Neuroglia - metabolism; Neuroglia - physiology; Neurons - metabolism; Signal Transduction - physiology; Spinal cord; Spinal Cord - cytology; Spinal Cord - embryology; Spinal Cord - metabolism; wnt7b; Spinal cord; BMP; BMP(s); Chick embryo; wnt7b; Development; CNS; Astrocyte; AP
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2010.05.017

In the vertebrate central nervous system (CNS), astrocytes are the most abundant and functionally diverse glial cell population. However, the mechanisms underlying their specification and differentiation are still poorly understood. In this study, we have defined spatially and temporally the origin of astrocytes and studied the role of BMPs in astrocyte development in the embryonic chick spinal cord. Using explant cultures, we show that astrocyte precursors started migrating out of the neuroepithelium in the mantle layer from E5, and that the dorsal-most level of the neuroepithelium, from the roof plate to the dl3 level, did not generate GFAP-positive astrocytes. Using a variety of early astrocyte markers together with functional analyses, we show that dorsal-most progenitors displayed a potential for astrocyte production but that dorsally-derived BMP signalling, possibly mediated through BMP receptor 1B, promoted neuronal specification instead. BMP treatment completely prevented astrocyte development from intermediate spinal cord explants at E5, whereas it promoted it at E6. Such an abrupt change in the response of this tissue to BMP signalling could be correlated to the onset of new foci of BMP activity and enhanced expression of BMP receptor 1A, suggesting that BMP signalling could promote astrocyte development in this region.