Effects of some drugs on purified human erythrocyte CuZnSOD and in vitro inhibitiory effect of 5-fluorouracil on leukocyte total SOD activity

• Published in: Journal of enzyme inhibition and medicinal chemistry Vol. 21; no. 2; p. 235
• Main Authors: Mavi, Ahmet, Küfrevioğlu, O Irfan, Yildirim, Ali
• Format: Journal Article
• Language: English
• Published: England 01-04-2006
• Subjects: Chromatography, Affinity; Chromatography, Ion Exchange; Enzyme Inhibitors - pharmacology; Erythrocytes - enzymology; Fluorouracil - pharmacology; Humans; In Vitro Techniques; Leukocytes - cytology; Leukocytes - enzymology; Leukocytes - metabolism; Superoxide Dismutase - antagonists & inhibitors; Superoxide Dismutase - isolation & purification; Superoxide Dismutase - metabolism
• ISSN: 1475-6366
• DOI: 10.1080/14756360500508416

The inhibition and activation effects of some drugs on the activities of superoxide dismutase enzymes (SOD) in human erythrocyte and leukocyte cells was investigated. Firstly, CuZnSOD enzyme was purified 837-fold and 12% efficiency from human erythrocytes by ethanol-chloroform treatment to remove hemoglobin and then ion exchange chromatography (DEAE-Sepharose) and copper chelate affinity chromatography techniques. Inhibition or activation effects of fourteen drugs on CuZnSOD was investigated. None of the studied drugs except for 5-fluorouracil showed any effects on the enzyme. 5-fluorouracil showed activation effects on CuZnSOD at 3.33mg/ml and 4mg/ml concentrations with 33% and 32% activation, respectively. Leukocytes were isolated from healthy human blood, lysed in liquid nitrogen and the effect of 5-fluorouracil on the lysate SOD activity investigated. 5-Fluorouracil showed inhibition effects on total SOD activity of human leukocytes at 2 mg/ml and 4 mg/ml concentrations with 42% and 62% inhibition, respectively.