Genome-wide identification and expression analysis of the PP2C gene family in Apocynum venetum and Apocynum hendersonii

• Published in: BMC plant biology Vol. 24; no. 1; pp. 652 - 16
• Main Authors: Chen, Jiayi, Wang, Yue, Wu, Yongmei, Huang, Xiaoyu, Qiu, Xiaojun, Chen, Jikang, Lin, Qian, Zhao, Haohan, Chen, Fengming, Gao, Gang
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09-07-2024; BioMed Central; BMC
• Subjects: Abiotic stress; Abscisic acid; Amino acids; Apocynum; Apocynum - genetics; Botanical research; Chromosomes; Desert environments; Ecological restoration; Environmental restoration; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Gene families; Genes; Genes, Plant; Genome, Plant; Genomes; Genomic analysis; Genomics; Harsh environments; Identification and classification; Kinases; Localization; Medicinal plants; Molecular weight; Multigene Family; Phosphatase; Phosphatases; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - metabolism; Phosphorylation; Phylogenetics; Phylogeny; Physiological aspects; Plant genetics; Plant growth; Plant Proteins - genetics; Plant Proteins - metabolism; Protein phosphatase; Protein Phosphatase 2C - genetics; Protein Phosphatase 2C - metabolism; Protein phosphatase 2 C; Proteins; Saline soils; Salinity tolerance; Salt stress; Salt tolerance; Salt Tolerance - genetics; Signal transduction; Soil improvement; Soil stresses; Subgroups; China; Protein phosphatase 2 C; Apocynum; Salt stress
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/s12870-024-05328-6

Protein phosphatase class 2 C (PP2C) is the largest protein phosphatase family in plants. Members of the PP2C gene family are involved in a variety of physiological pathways in plants, including the abscisic acid signalling pathway, the regulation of plant growth and development, etc., and are capable of responding to a wide range of biotic and abiotic stresses, and play an important role in plant growth, development, and response to stress. Apocynum is a perennial persistent herb, divided into Apocynum venetum and Apocynum hendersonii. It mainly grows in saline soil, deserts and other harsh environments, and is widely used in saline soil improvement, ecological restoration, textiles and medicine. A. hendersonii was found to be more tolerant to adverse conditions. The main purpose of this study was to investigate the PP2C gene family and its expression pattern under salt stress and to identify important candidate genes related to salt tolerance.
In this study, 68 AvPP2C genes and 68 AhPP2C genes were identified from the genomes of A. venetum and A. hendersonii, respectively. They were classified into 13 subgroups based on their phylogenetic relationships and were further analyzed for their subcellular locations, gene structures, conserved structural domains, and cis-acting elements. The results of qRT-PCR analyses of seven AvPP2C genes and seven AhPP2C genes proved that they differed significantly in gene expression under salt stress. It has been observed that the PP2C genes in A. venetum and A. hendersonii exhibit different expression patterns. Specifically, AvPP2C2, 6, 24, 27, 41 and AhPP2C2, 6, 24, 27, 42 have shown significant differences in expression under salt stress. This indicates that these genes may play a crucial role in the salt tolerance mechanism of A. venetum and A. hendersonii.
In this study, we conducted a genome-wide analysis of the AvPP2C and AhPP2C gene families in Apocynum, which provided a reference for further understanding the functional characteristics of these genes.