Trichoderma harzianum genes induced during growth on Rhizoctonia solani cell walls

• Published in: Microbiology (Society for General Microbiology) Vol. 141; no. 4; pp. 767 - 774
• Main Authors: Vasseur, Valerie, Van Montagu, Marc, Goldman, Gustavo H
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-04-1995; Society for General Microbiology
• Subjects: Amino Acid Sequence; Amino Acid Transport Systems; Base Sequence; Biological and medical sciences; Biological control; Cell Wall; Cloning, Molecular; Codon - genetics; Control; DNA, Fungal - genetics; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Fungal Proteins - genetics; Gene Expression Regulation, Fungal; Genes, Fungal; Genes. Genome; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Membrane Transport Proteins - genetics; Microbiology; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mycology; Phytopathology. Animal pests. Plant and forest protection; Plants - microbiology; Rhizoctonia - pathogenicity; Rhizoctonia solani; Sequence Homology, Amino Acid; Space life sciences; Trichoderma - enzymology; Trichoderma - genetics; Trichoderma - growth & development; Trichoderma harzianum; Plant pathogen; Induction; Biological control; Gene expression; Cell wall; Fungi; Mycoparasite; Proteins; Trichoderma harzianum; Antagonism; Rhizoctonia solani; Fungi Imperfecti; Comparative study; Aminoacid sequence; Thallophyta; Structural analysis
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/13500872-141-4-767

Laboratorium voor Genetica, Universiteit Gent K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
ABSTRACT
Trichoderma harzianum is a biocontrol agent that attacks a range of economically important phytopathogenic fungi. In an attempt to identify genes specifically expressed by T. harzianum during growth on cell walls of Rhizoctonia solani , we carried out differential screening of an induced cDNA library. In this paper we report the analysis of the sequence and expression of two cDNA clones that encode putative mycoparasitism-related proteins of T. harzianum . One of these clones corresponds to a gene, inda 1 , that encodes a protein of 570 amino acids with a predicted molecular mass of 62853 Da. The predicted amino acid sequence of inda1 showed a high degree of similarity with amino acid permeases from several other organisms. The other cDNA clone corresponds to a gene, indc11, that encodes a novel protein of 340 ami acids with a predicted molecular mass of 37010 Da. The use of this methodology should provide specific genetic markers to follow mycoparasitism by Trichoderma spp .
Author for correspondence: Marc Van Montagu. Tel: + 32 9 2645170. Fax: [phone]. e-mail: BITNET [e-mail]
The EMBL accession numbers for the inda 1 and indc11 nucleotide sequences reported in this paper are Z22594 and Z22221 , respectively.
Present address: Universidade de São Paulo, Faculdade de Ci ncias Farmac uticas de Ribeirão Preto, Via do Café S/N, 14040-903, Ribeirão Preto, SP, Brazil.
Present address: ESMISAB-Laboratoire de Microbiologie et Biochimie, Technopole Brest-lroise, F-29280, Plouzané, France.