Response of dynamic structure to removal of a disulfide bond: Normal mode refinement of C77A/C95A mutant of human lysozyme

• Published in: Protein science Vol. 3; no. 1; pp. 92 - 102
• Main Authors: Kidera, Akinori, Inaka, Koji, Matsushima, Masaaki, Gō, Nobuhiro
• Format: Journal Article
• Language: English
• Published: Bristol Cold Spring Harbor Laboratory Press 01-01-1994
• Subjects: Binding Sites; cavity‐creating mutation; Crystallization; Crystallography, X-Ray; disulfide mutant; Disulfides - chemistry; dynamic structure; human lysozyme; Humans; Models, Molecular; Molecular Structure; Muramidase - chemistry; Mutation; normal mode refinement; Structure-Activity Relationship; Thermodynamics
• ISSN: 0961-8368; 1469-896X
• DOI: 10.1002/pro.5560030112

In order to investigate the response of dynamic structure to removal of a disulfide bond, the dynamic structure of human lysozyme has been compared to its C77A/C95A mutant. The dynamic structures of the wild type and mutant are determined by normal mode refinement of 1.5‐Å‐resoltion X‐ray data. The C77AK95A mutant shows an increase in apparent fluctuations at most residues. However, most of the change originates from an increase in the external fluctuations, reflecting the effect of the mutation on the quality of crystals. The effects of disulfide bond removal on the internal fluctuations are almost exclusively limited to the mutation site at residue 77. No significant change in the correlation of the internal fluctuations is found in either the overall or local dynamics. This indicates that the disulfide bond does not have any substantial role to play in the dynamic structure. A comparison of the wild‐type and mutant coordinates suggests that the disulfide bond does not prevent the 2 domains from parting from each other. Instead, the structural changes are characteristic of a cavity‐creating mutation, where atoms surrounding the mutation site move cooperatively toward the space created by the smaller alanine side chain. Although this produces tighter packing, more than half of the cavity volume remains unoccupied, thus destabilizing the native state.