Fish collagen peptide inhibits the adipogenic differentiation of preadipocytes and ameliorates obesity in high fat diet-fed mice

• Published in: International journal of biological macromolecules Vol. 104; no. Pt A; pp. 281 - 286
• Main Authors: Lee, Eun Ji, Hur, Jinwoo, Ham, Sun Ah, Jo, Yeonji, Lee, SangYoon, Choi, Mi-Jung, Seo, Han Geuk
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-11-2017
• Subjects: 3T3-L1 Cells; Adipocytes - cytology; Adipocytes - drug effects; Adipogenesis; Adipogenesis - drug effects; Animals; Body Weight - drug effects; C/EBP-α; CCAAT-Enhancer-Binding Protein-alpha - metabolism; Cell Differentiation - drug effects; Cell Survival - drug effects; Collagen - chemistry; Diet, High-Fat - adverse effects; Fish collagen peptide; Gene Expression Regulation - drug effects; Hep G2 Cells; High fat diet; Humans; Lipids - blood; Male; Mice; Obesity - chemically induced; Obesity - drug therapy; Obesity - metabolism; Obesity - pathology; Peptide Fragments - pharmacology; Peptide Fragments - therapeutic use; PPAR gamma - metabolism; PPAR-γ; C/EBP-α; Fish collagen peptide; PPAR-γ; Adipogenesis; High fat diet
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2017.05.151

Bioactivities of fish collagen peptide are now being elucidated in diverse biological systems. Here, we investigated the effect of fish collagen peptide on the adipogenic differentiation of 3T3-L1 preadipocytes and in obese mice fed a high fat diet (HFD). Subcritical water-hydrolyzed fish collagen peptide (SWFCP) significantly inhibited lipid accumulation during the differentiation of 3T3-L1 preadipocytes, which was accompanied by decreased expression of CCAAT-enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), and adipocyte protein 2 (aP2) genes, key regulators of differentiation and maintenance of adipocytes. SWFCP was also found to suppress the palmitate-induced accumulation of lipid vacuoles in hepatocytes. Oral administration of SWFCP significantly reduced HFD-induced body weight gain without a significant difference in food intake. Consistent with its effects in 3T3-L1 preadipocytes, SWFCP inhibited the expression of C/EBP-α, PPAR-γ, and aP2 in epididymal adipose tissue of mice fed a HFD, leading to a significant reduction in adipocyte size. Furthermore, SWFCP significantly reduced serum levels of total cholesterol, triglyceride, and low-density lipoprotein, and increased serum high-density lipoprotein. These observations suggest that SWFCP inhibits adipocyte differentiation through a mechanism involving transcriptional repression of the major adipogenic regulators C/EBP-α and PPAR-γ, thereby reducing body weight gain and adipogenesis in an animal model of obesity.