Bone tissue response to titanium implant surfaces modified with carboxylate and sulfonate groups

• Published in: Journal of materials science. Materials in medicine Vol. 21; no. 2; pp. 707 - 715
• Main Authors: Kerner, S., Migonney, V., Pavon-Djavid, G., Helary, G., Sedel, L., Anagnostou, F.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01-02-2010; Springer; Springer Nature B.V
• Subjects: Alkanesulfonates - chemistry; Animals; Biochemistry; Biological and medical sciences; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biomedical research; Biomimetic Materials - chemistry; Bones; Carbon - chemistry; Ceramics; Chemistry and Materials Science; Coated Materials, Biocompatible - chemistry; Composites; Femur - cytology; Femur - surgery; Glass; Male; Materials Science; Materials Testing; Medical sciences; Natural Materials; Orthopedic surgery; Particle Size; Polymer Sciences; Prostheses and Implants; Rabbits; Regenerative Medicine/Tissue Engineering; Surface Properties; Surfaces and Interfaces; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology. Biomaterials. Equipments; Thin Films; Titanium - chemistry; Titanium alloys; Haversian Canal; Titanium Surface; Bone Formation; Carboxylate Content; Affect Bone Healing; Titanium alloy; Femur; Osteointegration; Implant; Rabbit; Polymer; Lagomorpha; Surface treatment; Sulfonate; Osteoarticular system; Vertebrata; Mammalia; Animal; Carboxylate; Biomaterial; Bone; Biomedical engineering
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-009-3928-5

The present study assessed in vivo new bone formation around titanium alloy implants chemically grafted with macromolecules bearing ionic sulfonate and/or carboxylate groups. Unmodified and grafted Ti–6Al–4V exhibiting either 100% carboxylate, or 100% sulfonate, or both carboxylate and sulfonate groups in the percent of 50/50 and 80/20 were bilaterally implanted into rabbit femoral condyle. Neither toxicity nor inflammation were observed for all implants tested. After 4 weeks, peri-implant new bone formation varied as a function of the chemical composition of the titanium surfaces. The percent bone-implant contact (BIC) was the lowest (13.4 ± 6.3%) for the implants modified with grafted carboxylate only. The value of BIC on the implants with 20% sulfonate (24.6 ± 5.2%) was significantly (
P
 < 0.05) lower than that observed on 100% sulfonate (38.2 ± 13.2%) surfaces. After both 4 and 12 weeks post-implantation, the BIC value for implants with more than 50% sulfonate was similar to that obtained with the unmodified Ti–6Al–4V. The grafted titanium alloy exhibiting either 100% sulfonate or carboxylate and sulfonate (50% each) groups promoted bone formation. Such materials are of clinical interest because, they do not promote bacteria adhesion but, they support new bone formation, a condition which can lead to osseointegration of bone implants while preventing peri-implant infections.