Effects of kinetin supplementation on the post-thaw motility, viability, and structural integrity of dog sperm

• Published in: Cryobiology Vol. 95; pp. 90 - 96
• Main Authors: Qamar, Ahmad Yar, Fang, Xun, Bang, Seonggyu, Kim, Min Jung, Cho, Jongki
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-08-2020
• Subjects: Animals; Cryopreservation; Cryopreservation - methods; Dietary Supplements; Dogs; Expression level; Humans; Kinetin; Male; Membrane Proteins; Mitochondrial Proteins; Motion characteristics; Oxidative stress; Semen Analysis; Semen Preservation - veterinary; Sperm Motility; Spermatozoa; Oxidative stress; Motion characteristics; Cryopreservation; Expression level
• ISSN: 0011-2240; 1090-2392
• DOI: 10.1016/j.cryobiol.2020.05.015

Oxidative stress is one of the major issues associated with cryopreservation because it causes a marked reduction in the post-thaw quality of semen. This study investigated the ability of kinetin to preserve the structural and functional integrity of dog sperm during cryopreservation. Pooled ejaculates were divided into 5 equal aliquots, diluted with buffer 2 supplemented with different concentrations of kinetin (0, 25, 50, 100, and 200 μM), and finally cryopreserved. The optimal concentration of kinetin was 50 μM based on the significantly improved (P < 0.05) motion characteristics and viability of post-thaw sperm samples. Moreover, kinetin-supplemented samples exhibited significantly higher (P < 0.05) sperm counts with the intact plasma membrane, normal acrosomes, mitochondria, and chromatin than control. The beneficial effects of kinetin were also reflected by the significant increase in the expression levels of anti-apoptotic (B-cell lymphoma, BCL2) and protamine-related genes (protamine 2, PRM2; protamine 3, PRM3), and decrease in the expression of pro-apoptotic (BCL2-associated X, BAX) and mitochondrial reactive oxygen species-modulating genes (ROS modulator 1, ROMO1) in kinetin-supplemented sperm samples than in control. The results demonstrated that supplementation of buffer 2 with 50 μM kinetin is ideal for reducing the magnitude of oxidative damage during semen cryoprocessing and improving the post-thaw quality of dog semen.
•Investigated the effects of kinetin supplementation on dog sperm cryopreservation.•Motion characteristics, viability, and structural integrity of sperm were assessed.•Kinetin 50 μM resulted in higher quality of freeze-thaw sperm than other groups.•Kinetin positively affects the gene expression in dog sperm.