Competitive Multiplication of RNA3 Species of Different Strains of Alfalfa Mosaic Virus

• Published in: Journal of general virology Vol. 70; no. 3; pp. 777 - 782
• Main Authors: Dore, Jean-Michel, Pinck, Monique, Pinck, Lothaire
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-03-1989; Society for General Microbiology
• Subjects: alfalfa mosaic virus; Amino Acid Sequence; Base Sequence; Biological and medical sciences; DNA - genetics; DNA, Viral - genetics; Fundamental and applied biological sciences. Psychology; Genes, Viral; Genetics; Medicago sativa - microbiology; Microbiology; Mosaic Viruses - genetics; Mosaic Viruses - physiology; RNA, Viral - genetics; Transcription, Genetic; Virology; Virus Replication; Virus; Competition; Inoculation; Leader sequence; RNA; Gel electrophoresis; Alfalfa mosaic virus; Replication; Phytopathogen; Nucleic acid; Comparative study; Strain
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-70-3-777

Institut de Biologie Moléculaire des Plantes du CNRS, Laboratoire de Virologie, 12 rue du Général Zimmer, 67000 Strasbourg, France
Competition between RNA3 from alfalfa mosaic virus (AlMV) strain S (RNA3-S), strain B (RNA3-B) and strain 425L (RNA3-L) was studied. The identification of the RNA3 species multiplying in infected leaves was possible since the RNA3 5' noncoding leader sequences in strains S, B and 425L differ in length. RNA3 present in total RNA from infected tobacco leaves was detected, and strains were identified from the length of the cDNA reverse-transcribed from RNA primed with a specific oligonucleotide. In competition experiments the inoculum, containing known amounts of RNA1, 2, 3 and 4 of one strain, was complemented with various amounts of heterologous RNA3 and inoculated to a systemic host. It is shown that RNA3-S was better replicated in vivo by the AlMV replicase of strain B than was RNA3-B itself, and to a lesser extent better replicated by the AlMV replicase of strain L than was RNA3-L. Comparison of genetic information carried by the RNA3 species present in the inoculum suggests that the more efficient multiplication of RNA3-S is related to the structure of the leader sequence of RNA3-S.
Keywords: AlMV, replication, oligonucleotide priming
Received 12 July 1988;
accepted 24 October 1988.