Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina)

• Published in: International journal of systematic and evolutionary microbiology Vol. 60; no. Pt 4; pp. 972 - 976
• Main Authors: Kurilenko, Valerie V, Christen, Richard, Zhukova, Natalia V, Kalinovskaya, Nataliya I, Mikhailov, Valery V, Crawford, Russell J, Ivanova, Elena P
• Format: Journal Article
• Language: English
• Published: England Microbiology Society 01-04-2010
• Subjects: Bacterial Typing Techniques; Base Composition; Development Biology; DNA, Ribosomal - analysis; DNA, Ribosomal - genetics; Fatty Acids - analysis; Gammaproteobacteria - classification; Gammaproteobacteria - genetics; Gammaproteobacteria - isolation & purification; Gammaproteobacteria - physiology; Genes, rRNA; Genotype; Life Sciences; Molecular Sequence Data; Nucleic Acid Hybridization; Phenotype; Phylogeny; Plant Leaves - microbiology; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Species Specificity; Zosteraceae - microbiology
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.013516-0

A non-pigmented, motile, Gram-negative bacterium, strain Z 271(T), was isolated from the surface of leaves of the seagrass Zostera marina which was collected in Troitza Bay (Sea of Japan, Pacific Ocean). The new isolate grew between 5 degrees C and 28 degrees C and was slightly halophilic, tolerating environments containing up to 5 % (w/v) NaCl. Strain Z 271(T) was able to degrade Tweens 20, 40 and 80 and partially degrade gelatin, but was unable to degrade casein. Phosphatidylethanolamine (36.9 %) and phosphatidylglycerol (63.1 %) were the predominant phospholipids. The major fatty acids included C(18 : 1)omega7c (43.7 %), C(16 : 1)omega7c (31.1 %) and C(16 : 0) (16.8 %). The main respiratory quinone was Q-8. The DNA-DNA relatedness value of strain Z 271(T) with Granulosicoccus antarcticus IMCC3135(T) was 35 %. The G+C content of the DNA of strain Z 271(T) was 60.2 mol%. On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, strain Z 271(T) represents a novel species of the genus Granulosicoccus for which the name Granulosicoccus coccoides sp. nov. is proposed. The type strain is Z 271(T) (=KMM 6014(T)=CIP 109923(T)).