Isolation and characterization of Citrobacter spp. from the intestine of grass carp Ctenopharyngodon idellus

• Published in: Aquaculture Vol. 313; no. 1; pp. 156 - 160
• Main Authors: Lü, Aijun, Hu, Xiucai, Zheng, Lu, Zhu, Aihua, Cao, Chengliang, Jiang, Jihong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15-03-2011; Amsterdam: Elsevier Science; Elsevier; Elsevier Sequoia S.A
• Subjects: 16S rDNA; Animal and plant ecology; Animal aquaculture; Animal diseases; Animal productions; Animal, plant and microbial ecology; Aquaculture; bacteria; Bacterial infections; Biochemistry; Biological and medical sciences; Carp; Citrobacter; Citrobacter freundii; Citrobacter gillenii; Citrobacter werkmanii; Ctenopharyngodon idella; Ctenopharyngodon idellus; Danio rerio; Digestive system; fimC; fish; Fresh water ecosystems; Freshwater; Fundamental and applied biological sciences. Psychology; gastroenteritis; General aspects; Grass carp; intestinal microorganisms; intestines; mice; Pathogenesis; pathogenicity; Polymorphism; single-stranded conformational polymorphism; Studies; Synecology; China; China, People's Rep; Ctenopharyngodon idellus; Grass carp; 16S rDNA; fimC; Citrobacter; Characterization; Freshwater environment; Vertebrata; Ctenopharyngodon idella; Pisces; Bacteria; 16SrDNA fimC; Enterobacteriaceae; Aquaculture
• ISSN: 0044-8486; 1873-5622
• DOI: 10.1016/j.aquaculture.2011.01.018

Citrobacter gastroenteritis became an increasingly significant health problem in intensive aquaculture in China. In this study, three bacterial strains were isolated from the intestinal tract of farmed grass carp (
Ctenopharyngodon idellus), and were tentatively numbered C9414, C0520 and C0934, and then they were identified as
Citrobacter freundii,
C. gillenii and
C. werkmanii by the biochemical properties and molecular techniques. The strain C9414, namely
C. freundii showed the pathogenicity to mice and zebrafish, respectively. Furthermore, the fingerprint patterns with distinct bands of three isolates were generated by single-strand conformation polymorphism (SSCP) analysis. To our knowledge, this is the first report on the bacteria
Citrobacter spp. isolated from the intestine of fish in China.